Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3898345..3899039 Replicon chromosome
Accession NZ_CP098206
Organism Escherichia coli strain Z0117EC0054

Toxin (Protein)


Gene name yafO Uniprot ID S1PJQ2
Locus tag NBY18_RS19150 Protein ID WP_001263500.1
Coordinates 3898345..3898743 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag NBY18_RS19155 Protein ID WP_000554758.1
Coordinates 3898746..3899039 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NBY18_RS19125 (3893710) 3893710..3894168 - 459 WP_001291988.1 xanthine phosphoribosyltransferase -
NBY18_RS19130 (3894429) 3894429..3895886 + 1458 WP_001292999.1 cytosol nonspecific dipeptidase -
NBY18_RS19135 (3895943) 3895943..3896557 - 615 WP_000602123.1 peptide chain release factor H -
NBY18_RS19140 (3896554) 3896554..3897693 - 1140 WP_000521577.1 RNA ligase RtcB family protein -
NBY18_RS19145 (3897883) 3897883..3898335 - 453 WP_001059895.1 GNAT family N-acetyltransferase -
NBY18_RS19150 (3898345) 3898345..3898743 - 399 WP_001263500.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
NBY18_RS19155 (3898746) 3898746..3899039 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
NBY18_RS19160 (3899091) 3899091..3900146 - 1056 WP_001226168.1 DNA polymerase IV -
NBY18_RS19165 (3900217) 3900217..3901002 - 786 WP_000207544.1 putative lateral flagellar export/assembly protein LafU -
NBY18_RS19170 (3900974) 3900974..3902686 + 1713 Protein_3759 flagellar biosynthesis protein FlhA -
NBY18_RS19175 (3902765) 3902765..3902923 + 159 WP_014639450.1 hypothetical protein -
NBY18_RS19180 (3903006) 3903006..3903503 - 498 WP_000006241.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 3898345..3918699 20354


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15413.85 Da        Isoelectric Point: 8.9511

>T247067 WP_001263500.1 NZ_CP098206:c3898743-3898345 [Escherichia coli]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDAAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT247067 WP_000554758.1 NZ_CP098206:c3899039-3898746 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PJQ2

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References