Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 3655925..3656604 Replicon chromosome
Accession NZ_CP098206
Organism Escherichia coli strain Z0117EC0054

Toxin (Protein)


Gene name higB Uniprot ID V0VBP6
Locus tag NBY18_RS18005 Protein ID WP_000057524.1
Coordinates 3656302..3656604 (-) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag NBY18_RS18000 Protein ID WP_000806442.1
Coordinates 3655925..3656266 (-) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NBY18_RS17990 (3652169) 3652169..3653101 - 933 WP_000883052.1 glutaminase A -
NBY18_RS17995 (3653363) 3653363..3655867 + 2505 WP_000083945.1 copper-exporting P-type ATPase CopA -
NBY18_RS18000 (3655925) 3655925..3656266 - 342 WP_000806442.1 HigA family addiction module antitoxin Antitoxin
NBY18_RS18005 (3656302) 3656302..3656604 - 303 WP_000057524.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
NBY18_RS18010 (3656737) 3656737..3657531 + 795 WP_000365177.1 TraB/GumN family protein -
NBY18_RS18015 (3657735) 3657735..3658214 + 480 WP_000186633.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
NBY18_RS18020 (3658238) 3658238..3659038 + 801 WP_000439796.1 hypothetical protein -
NBY18_RS18025 (3659035) 3659035..3659538 + 504 WP_000667000.1 hypothetical protein -
NBY18_RS18030 (3659576) 3659576..3661228 - 1653 WP_000771762.1 bifunctional UDP-sugar hydrolase/5'-nucleotidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3648614..3659538 10924


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11839.47 Da        Isoelectric Point: 10.2237

>T247065 WP_000057524.1 NZ_CP098206:c3656604-3656302 [Escherichia coli]
MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHWDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT247065 WP_000806442.1 NZ_CP098206:c3656266-3655925 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0VBP6

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References