Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 4489242..4490041 Replicon chromosome
Accession NZ_CP098201
Organism Escherichia coli strain Z0117EC0056

Toxin (Protein)


Gene name yhaV Uniprot ID F0JQM9
Locus tag NBY20_RS21570 Protein ID WP_000347270.1
Coordinates 4489577..4490041 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID A0A0V9H1L4
Locus tag NBY20_RS21565 Protein ID WP_001551693.1
Coordinates 4489242..4489577 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NBY20_RS21550 (4485027) 4485027..4485797 - 771 WP_001551692.1 2-dehydro-3-deoxyglucarate aldolase -
NBY20_RS21555 (4485813) 4485813..4487147 - 1335 WP_000599651.1 galactarate/glucarate/glycerate transporter GarP -
NBY20_RS21560 (4487522) 4487522..4489093 + 1572 WP_001273738.1 galactarate dehydratase -
NBY20_RS21565 (4489242) 4489242..4489577 + 336 WP_001551693.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
NBY20_RS21570 (4489577) 4489577..4490041 + 465 WP_000347270.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
NBY20_RS21575 (4490096) 4490096..4490905 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
NBY20_RS21580 (4491154) 4491154..4492434 + 1281 WP_001551694.1 tagatose-bisphosphate aldolase subunit KbaZ -
NBY20_RS21585 (4492457) 4492457..4492930 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
NBY20_RS21590 (4492941) 4492941..4493720 + 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
NBY20_RS21595 (4493710) 4493710..4494588 + 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
NBY20_RS21600 (4494606) 4494606..4495040 + 435 WP_000948837.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4480480..4490041 9561


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17806.22 Da        Isoelectric Point: 9.6924

>T247021 WP_000347270.1 NZ_CP098201:4489577-4490041 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEEAH

Download         Length: 465 bp

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12347.87 Da        Isoelectric Point: 4.6890

>AT247021 WP_001551693.1 NZ_CP098201:4489242-4489577 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDTQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0V9NQ90

Antitoxin

Source ID Structure
AlphaFold DB A0A0V9H1L4

References