Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3915819..3916546 Replicon chromosome
Accession NZ_CP098201
Organism Escherichia coli strain Z0117EC0056

Toxin (Protein)


Gene name higB Uniprot ID V0YLE2
Locus tag NBY20_RS18805 Protein ID WP_000547555.1
Coordinates 3916235..3916546 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag NBY20_RS18800 Protein ID WP_000126294.1
Coordinates 3915819..3916238 (-) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NBY20_RS18780 (3910904) 3910904..3911431 - 528 WP_001078777.1 electron transport protein HydN -
NBY20_RS18785 (3911580) 3911580..3912590 - 1011 WP_029130985.1 DNA-binding transcriptional regulator AscG -
NBY20_RS18790 (3912850) 3912850..3914307 + 1458 WP_001107889.1 PTS cellobiose/arbutin/salicin transporter subunit IIBC -
NBY20_RS18795 (3914316) 3914316..3915740 + 1425 WP_000110355.1 6-phospho-beta-glucosidase AscB -
NBY20_RS18800 (3915819) 3915819..3916238 - 420 WP_000126294.1 helix-turn-helix domain-containing protein Antitoxin
NBY20_RS18805 (3916235) 3916235..3916546 - 312 WP_000547555.1 type II toxin-antitoxin system HigB family toxin Toxin
NBY20_RS18810 (3916713) 3916713..3917183 - 471 WP_001551542.1 hydrogenase maturation peptidase HycI -
NBY20_RS18815 (3917176) 3917176..3917586 - 411 WP_001291918.1 formate hydrogenlyase assembly protein HycH -
NBY20_RS18820 (3917583) 3917583..3918350 - 768 WP_000067399.1 formate hydrogenlyase subunit HycG -
NBY20_RS18825 (3918350) 3918350..3918892 - 543 WP_000493785.1 formate hydrogenlyase subunit HycF -
NBY20_RS18830 (3918902) 3918902..3920611 - 1710 WP_001288122.1 formate hydrogenlyase subunit HycE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12439.18 Da        Isoelectric Point: 9.5146

>T247017 WP_000547555.1 NZ_CP098201:c3916546-3916235 [Escherichia coli]
MHIISKAPFEECARKYPNDALALHSLYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Download         Length: 312 bp

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15416.42 Da        Isoelectric Point: 4.4596

>AT247017 WP_000126294.1 NZ_CP098201:c3916238-3915819 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Download         Length: 420 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0YLE2

Antitoxin

Source ID Structure

References