Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1268068..1268795 Replicon chromosome
Accession NZ_CP098192
Organism Escherichia coli strain Z0117EC0116

Toxin (Protein)


Gene name higB Uniprot ID J7Q991
Locus tag NBY24_RS06230 Protein ID WP_000547564.1
Coordinates 1268068..1268379 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag NBY24_RS06235 Protein ID WP_000126294.1
Coordinates 1268376..1268795 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NBY24_RS06200 (1263210) 1263210..1264919 + 1710 WP_001288134.1 formate hydrogenlyase subunit HycE -
NBY24_RS06205 (1264929) 1264929..1265471 + 543 WP_000493792.1 formate hydrogenlyase subunit HycF -
NBY24_RS06210 (1265471) 1265471..1266238 + 768 WP_000067399.1 formate hydrogenlyase subunit HycG -
NBY24_RS06215 (1266235) 1266235..1266645 + 411 WP_001291921.1 formate hydrogenlyase assembly protein HycH -
NBY24_RS06220 (1266638) 1266638..1267108 + 471 WP_000132961.1 hydrogenase maturation peptidase HycI -
NBY24_RS06225 (1267133) 1267133..1267894 + 762 WP_001026446.1 hypothetical protein -
NBY24_RS06230 (1268068) 1268068..1268379 + 312 WP_000547564.1 type II toxin-antitoxin system HigB family toxin Toxin
NBY24_RS06235 (1268376) 1268376..1268795 + 420 WP_000126294.1 helix-turn-helix domain-containing protein Antitoxin
NBY24_RS06240 (1268909) 1268909..1270333 - 1425 WP_000110363.1 6-phospho-beta-glucosidase AscB -
NBY24_RS06245 (1270342) 1270342..1271799 - 1458 WP_250780765.1 PTS cellobiose/arbutin/salicin transporter subunit IIBC -
NBY24_RS06250 (1272059) 1272059..1273069 + 1011 WP_001392554.1 DNA-binding transcriptional regulator AscG -
NBY24_RS06255 (1273218) 1273218..1273745 + 528 WP_001078777.1 electron transport protein HydN -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12426.13 Da        Isoelectric Point: 9.7248

>T246913 WP_000547564.1 NZ_CP098192:1268068-1268379 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Download         Length: 312 bp

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15416.42 Da        Isoelectric Point: 4.4596

>AT246913 WP_000126294.1 NZ_CP098192:1268376-1268795 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Download         Length: 420 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A0FJG5

Antitoxin

Source ID Structure

References