Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 12270..12916 Replicon plasmid pZ0117EC0148-4
Accession NZ_CP098182
Organism Escherichia coli strain Z0117EC0148

Toxin (Protein)


Gene name higB Uniprot ID -
Locus tag NBY28_RS25175 Protein ID WP_000269912.1
Coordinates 12270..12617 (+) Length 116 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A1V3UZT0
Locus tag NBY28_RS25180 Protein ID WP_001259436.1
Coordinates 12617..12916 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NBY28_RS25150 (NBY28_25150) 8690..9013 - 324 WP_250844260.1 hypothetical protein -
NBY28_RS25155 (NBY28_25155) 9095..9649 + 555 WP_033561046.1 recombinase family protein -
NBY28_RS25160 (NBY28_25160) 9831..10808 + 978 WP_224922639.1 plasmid replication initiator RepA -
NBY28_RS25165 (NBY28_25165) 11452..11739 + 288 WP_000356589.1 hypothetical protein -
NBY28_RS25170 (NBY28_25170) 11763..12026 + 264 WP_000424604.1 hypothetical protein -
NBY28_RS25175 (NBY28_25175) 12270..12617 + 348 WP_000269912.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
NBY28_RS25180 (NBY28_25180) 12617..12916 + 300 WP_001259436.1 XRE family transcriptional regulator Antitoxin
NBY28_RS25185 (NBY28_25185) 13079..13459 + 381 WP_111982070.1 hypothetical protein -
NBY28_RS25190 (NBY28_25190) 13523..13795 + 273 WP_000148348.1 helix-turn-helix domain-containing protein -
NBY28_RS25195 (NBY28_25200) 14605..15747 - 1143 WP_089592802.1 ORF6N domain-containing protein -
NBY28_RS25200 (NBY28_25205) 15744..15935 - 192 WP_089592803.1 hypothetical protein -
NBY28_RS25210 (NBY28_25215) 16385..16978 + 594 WP_250844261.1 helix-turn-helix domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..52058 52058


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-105)

Antitoxin

Cro/C1-type helix-turn-helix domain

(32-80)


Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 13545.51 Da        Isoelectric Point: 8.8337

>T246854 WP_000269912.1 NZ_CP098182:12270-12617 [Escherichia coli]
MWTVLFSQRFDDWLNEQEDALQEKVLADLKKLQVYGPELPRPYADTVKGSRYKNMKELRVQFSGRPIRAFYAFDPIRRAI
VLCAGDKSNDKRFYEKLVRIAEDEFAAHLNTLESK

Download         Length: 348 bp

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 11074.92 Da        Isoelectric Point: 5.2090

>AT246854 WP_001259436.1 NZ_CP098182:12617-12916 [Escherichia coli]
MRTLDEVIASRSPESQARIKEMADEMILEVGLQMMREELQLSQKQVAEAMGISQPAVTKLEQRGNDLKLATLKRYVEAMG
GKLSLDVELPTGKRIAFNI

Download         Length: 300 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A1V3UZT0

References