Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 4176395..4177194 Replicon chromosome
Accession NZ_CP098178
Organism Escherichia coli strain Z0117EC0148

Toxin (Protein)


Gene name yhaV Uniprot ID U9XVR9
Locus tag NBY28_RS20355 Protein ID WP_000347267.1
Coordinates 4176730..4177194 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag NBY28_RS20350 Protein ID WP_001307405.1
Coordinates 4176395..4176730 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NBY28_RS20335 (4172180) 4172180..4172950 - 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -
NBY28_RS20340 (4172966) 4172966..4174300 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
NBY28_RS20345 (4174675) 4174675..4176246 + 1572 WP_001273741.1 galactarate dehydratase -
NBY28_RS20350 (4176395) 4176395..4176730 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
NBY28_RS20355 (4176730) 4176730..4177194 + 465 WP_000347267.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
NBY28_RS20360 (4177249) 4177249..4178058 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
NBY28_RS20365 (4178307) 4178307..4179587 + 1281 WP_000681921.1 tagatose-bisphosphate aldolase subunit KbaZ -
NBY28_RS20370 (4179610) 4179610..4180083 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
NBY28_RS20375 (4180094) 4180094..4180873 + 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
NBY28_RS20380 (4180863) 4180863..4181741 + 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
NBY28_RS20385 (4181759) 4181759..4182193 + 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4165407..4177194 11787


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17807.17 Da        Isoelectric Point: 9.4947

>T246838 WP_000347267.1 NZ_CP098178:4176730-4177194 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT246838 WP_001307405.1 NZ_CP098178:4176395-4176730 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XTR4

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References