Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/RelE-HTH
Location 2534981..2535670 Replicon chromosome
Accession NZ_CP098167
Organism Klebsiella pneumoniae strain Z0117KP0004

Toxin (Protein)


Gene name higB Uniprot ID A0A331C6E2
Locus tag NBY32_RS12440 Protein ID WP_021469727.1
Coordinates 2534981..2535298 (+) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A6B0N7G3
Locus tag NBY32_RS12445 Protein ID WP_020804705.1
Coordinates 2535374..2535670 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NBY32_RS12410 (2530683) 2530683..2531192 + 510 WP_020802144.1 GNAT family N-acetyltransferase -
NBY32_RS12415 (2531202) 2531202..2532128 + 927 WP_032435210.1 amino acid ABC transporter permease -
NBY32_RS12420 (2532112) 2532112..2532891 + 780 WP_002906697.1 amino acid ABC transporter ATP-binding protein -
NBY32_RS12425 (2532930) 2532930..2533781 + 852 WP_072198177.1 transporter substrate-binding domain-containing protein -
NBY32_RS12430 (2533859) 2533859..2534476 + 618 WP_032425015.1 glutathione S-transferase family protein -
NBY32_RS12435 (2534547) 2534547..2534774 + 228 WP_002906690.1 tautomerase PptA -
NBY32_RS12440 (2534981) 2534981..2535298 + 318 WP_021469727.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
NBY32_RS12445 (2535374) 2535374..2535670 + 297 WP_020804705.1 NadS family protein Antitoxin
NBY32_RS12450 (2535749) 2535749..2536195 + 447 WP_032435212.1 hypothetical protein -
NBY32_RS12455 (2536236) 2536236..2537852 - 1617 WP_004175961.1 carbohydrate porin -
NBY32_RS12460 (2537896) 2537896..2539278 - 1383 WP_004151222.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-2

(43-102)

Antitoxin

Cro/C1-type helix-turn-helix domain

(39-75)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12221.10 Da        Isoelectric Point: 10.2217

>T246780 WP_021469727.1 NZ_CP098167:2534981-2535298 [Klebsiella pneumoniae]
MLTFIELQGFSKRRQVLLQDDEFRAFQEILINDPEAGDTISGTGGFRKIRWSRPGMGKRGGVRVIYYNVTKKGRIYLALI
YPKNEQDELNQEQKKMLRQVADLIN

Download         Length: 318 bp

>T246780 NZ_CP098167:2534981-2535298 [Klebsiella pneumoniae]
ATGTTGACTTTTATTGAACTACAAGGTTTCAGTAAGCGACGCCAGGTGCTGTTGCAGGATGATGAATTTCGGGCCTTTCA
GGAAATCCTTATAAACGATCCCGAGGCTGGGGACACAATTTCGGGAACCGGTGGTTTTCGTAAAATTCGCTGGAGTCGTC
CCGGGATGGGGAAGCGCGGCGGTGTACGAGTGATTTACTATAATGTAACGAAAAAGGGAAGAATTTACCTGGCGCTCATC
TATCCTAAAAATGAACAGGATGAGTTAAATCAGGAGCAAAAAAAGATGTTGAGACAAGTGGCTGATTTAATAAACTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11057.93 Da        Isoelectric Point: 6.7277

>AT246780 WP_020804705.1 NZ_CP098167:2535374-2535670 [Klebsiella pneumoniae]
MKDELFADLLASAEEMVRIEKGEQMLMEQHVHTFSEIDVKAIREATGLKQQDFAAAVGVSYELVKSWETKRRHPTGAARK
LLLLLQGNPLIINLLKVV

Download         Length: 297 bp

>AT246780 NZ_CP098167:2535374-2535670 [Klebsiella pneumoniae]
ATGAAAGACGAATTGTTTGCCGATCTGCTGGCGAGTGCAGAAGAAATGGTCAGGATCGAGAAAGGTGAACAAATGCTGAT
GGAGCAGCACGTTCATACCTTCAGCGAAATTGACGTGAAAGCGATACGTGAAGCCACAGGTTTAAAACAGCAGGACTTTG
CAGCGGCCGTTGGCGTTAGCTACGAGTTGGTTAAAAGCTGGGAAACGAAACGACGTCACCCAACCGGTGCAGCCAGAAAG
CTGCTTTTATTGCTTCAGGGTAATCCATTGATTATCAACTTGTTGAAGGTTGTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A331C6E2

Antitoxin

Source ID Structure
AlphaFold DB A0A6B0N7G3

References