Detailed information of TA system TA024667

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1136258..1136851
• Replicon: chromosome
• Accession: NC_013960
• Organism: Nitrosococcus halophilus Nc 4

Toxin (Protein)

• Gene name: higB
• Uniprot ID: D5BZL7
• Locus tag: NHAL_RS05670
• Protein ID: WP_013032204.1
• Coordinates: 1136258..1136536 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: NHAL_RS05675
• Protein ID: WP_013032205.1
• Coordinates: 1136549..1136851 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NHAL_RS05645	1131551..1131961	+	411	WP_013032200.1	hypothetical protein	-
NHAL_RS05650	1131939..1132283	+	345	WP_041354671.1	hypothetical protein	-
NHAL_RS05655	1132517..1133971	+	1455	WP_013032202.1	catalase	-
NHAL_RS05660	1134367..1134564	+	198	WP_013032203.1	hypothetical protein	-
NHAL_RS21475	1134582..1134908	-	327	WP_203434362.1	hypothetical protein	-
NHAL_RS21480	1135164..1135865	-	702	WP_203434363.1	Fic family protein	-
NHAL_RS05670	1136258..1136536	+	279	WP_013032204.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
NHAL_RS05675	1136549..1136851	+	303	WP_013032205.1	HigA family addiction module antidote protein	Antitoxin
NHAL_RS05680	1136955..1138178	-	1224	WP_013031487.1	IS256 family transposase	-
NHAL_RS05685	1138389..1140899	-	2511	WP_013032206.1	EAL domain-containing protein	-
NHAL_RS05690	1141138..1141623	-	486	WP_203434364.1	septal ring lytic transglycosylase RlpA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (6-83)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10738.23 Da
• Isoelectric Point: 10.4500

>T24667 WP_013032204.1 NC_013960:1136258-1136536 [Nitrosococcus halophilus Nc 4]
MIKSFRCQETQRLFETGKSRRWQSIRKVAERKLVQLNNARELRDLASSPGNQLEALEGSRAGQYSIRINKQWRVCFIWTS
EGPEAVEIVGYH

Nucleotide

• Length: 279 bp

>T24667 NC_013960:1136258-1136536 [Nitrosococcus halophilus Nc 4]
ATGATTAAAAGCTTCCGCTGCCAAGAGACCCAACGTCTGTTTGAAACCGGCAAATCCCGCCGGTGGCAAAGCATTCGCAA
GGTGGCAGAGCGCAAACTCGTTCAATTGAACAATGCGCGGGAGCTACGGGACCTAGCCTCTTCGCCAGGCAACCAACTGG
AAGCATTGGAAGGCAGCCGAGCTGGACAATACAGTATCCGAATCAATAAGCAGTGGCGGGTGTGCTTTATCTGGACGAGC
GAGGGACCGGAAGCCGTTGAAATTGTGGGCTATCATTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11535.41 Da
• Isoelectric Point: 9.6206

>AT24667 WP_013032205.1 NC_013960:1136549-1136851 [Nitrosococcus halophilus Nc 4]
MFKNGMRPVHPGEILREDYLVPLEMSVNALAKALHVPTPRINEIVRERRGISADTALRLARYFDTTPQFWLGLQAEYDLR
VAEIKEAKRIISEIQPRHAV

Nucleotide

• Length: 303 bp

>AT24667 NC_013960:1136549-1136851 [Nitrosococcus halophilus Nc 4]
ATGTTTAAAAATGGGATGCGCCCAGTTCATCCTGGAGAAATTTTGCGCGAGGACTATCTTGTGCCGCTTGAAATGAGCGT
TAACGCGTTGGCGAAAGCGCTTCATGTGCCCACGCCGCGCATTAATGAGATTGTGCGGGAGAGGCGTGGGATTAGCGCGG
ATACCGCGTTGCGATTAGCCCGCTATTTTGACACCACCCCTCAATTCTGGCTGGGACTACAAGCCGAGTACGACCTGCGC
GTTGCTGAAATAAAAGAAGCCAAACGTATTATCAGCGAAATCCAGCCGCGGCATGCTGTTTAA

Structures

Toxin

• AlphaFold DB: D5BZL7