Detailed information of TA system TA024661

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 213344..213934
• Replicon: chromosome
• Accession: NC_013960
• Organism: Nitrosococcus halophilus Nc 4

Toxin (Protein)

• Gene name: higB
• Uniprot ID: D5C4Y6
• Locus tag: NHAL_RS01025
• Protein ID: WP_013031305.1
• Coordinates: 213656..213934 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: NHAL_RS01020
• Protein ID: WP_013031304.1
• Coordinates: 213344..213646 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NHAL_RS01005	209420..211024	-	1605	WP_013031303.1	hypothetical protein	-
NHAL_RS01010	211499..212854	-	1356	WP_083761341.1	hypothetical protein	-
NHAL_RS21370	212761..212949	-	189	WP_041354533.1	hypothetical protein	-
NHAL_RS01020	213344..213646	-	303	WP_013031304.1	HigA family addiction module antidote protein	Antitoxin
NHAL_RS01025	213656..213934	-	279	WP_013031305.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
NHAL_RS01030	214382..215257	-	876	WP_013031306.1	tetratricopeptide repeat protein	-
NHAL_RS01035	215591..216466	-	876	WP_013031307.1	dihydroorotate dehydrogenase electron transfer subunit	-
NHAL_RS01040	216463..217752	-	1290	WP_013031308.1	dihydroorotase	-
NHAL_RS01045	217752..218726	-	975	WP_013031309.1	aspartate carbamoyltransferase catalytic subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (18-66)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10403.81 Da
• Isoelectric Point: 9.0259

>T24661 WP_013031305.1 NC_013960:c213934-213656 [Nitrosococcus halophilus Nc 4]
MIKGFKHKGLERFFLHGTKAGIQTKHTERLRLILGRLNASTCPQDMNLPGLGLHELSGDRKGIWSVKVSSNWRVTFTFSG
GDADDVDYEDYH

Nucleotide

• Length: 279 bp

>T24661 NC_013960:c213934-213656 [Nitrosococcus halophilus Nc 4]
ATGATCAAGGGCTTCAAACATAAAGGCCTGGAACGGTTCTTTCTCCACGGAACAAAGGCAGGCATTCAAACTAAGCATAC
TGAACGTTTGCGTTTGATTCTAGGTCGTTTGAACGCCTCTACATGCCCTCAAGACATGAACCTACCTGGATTAGGTCTAC
ATGAATTGAGCGGCGATCGCAAAGGCATTTGGTCTGTGAAGGTCAGTAGTAACTGGCGAGTCACCTTCACTTTCTCAGGT
GGAGATGCCGACGATGTGGACTATGAAGATTATCATTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11196.88 Da
• Isoelectric Point: 9.6889

>AT24661 WP_013031304.1 NC_013960:c213646-213344 [Nitrosococcus halophilus Nc 4]
MQMHNPPHPGEILKHLCLEPLGLSVTEAAKALGVSRKTLSSILNGRTGISPEMAIRLSIAFDTTAESWLNQQVQYDLWRA
EQHRGKLQVKKLSSKQSEYT

Nucleotide

• Length: 303 bp

>AT24661 NC_013960:c213646-213344 [Nitrosococcus halophilus Nc 4]
ATGCAGATGCACAACCCTCCCCATCCTGGTGAAATCCTAAAGCACCTGTGCCTTGAACCTTTGGGTTTAAGTGTTACTGA
AGCCGCCAAGGCGCTTGGGGTTAGCCGAAAGACTTTATCGAGCATTTTAAATGGCCGTACGGGTATTAGCCCTGAGATGG
CTATTCGTCTTTCTATTGCCTTTGATACAACAGCGGAAAGCTGGTTAAATCAGCAAGTTCAGTATGATCTCTGGCGGGCT
GAACAGCACCGTGGCAAACTTCAAGTAAAGAAGTTGTCATCCAAGCAATCTGAATATACCTAA

Structures

Toxin

• AlphaFold DB: D5C4Y6