Detailed information of TA system TA024656

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2223483..2224063
• Replicon: chromosome
• Accession: NC_013959
• Organism: Sideroxydans lithotrophicus ES-1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: D5CUN5
• Locus tag: SLIT_RS10965
• Protein ID: WP_013030320.1
• Coordinates: 2223785..2224063 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: SLIT_RS10960
• Protein ID: WP_013030319.1
• Coordinates: 2223483..2223785 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SLIT_RS10945	2220314..2222128	+	1815	WP_013030316.1	NERD domain-containing protein	-
SLIT_RS10950	2222236..2222736	+	501	WP_013030317.1	hypothetical protein	-
SLIT_RS10955	2222838..2223209	+	372	WP_013030318.1	hypothetical protein	-
SLIT_RS10960	2223483..2223785	-	303	WP_013030319.1	HigA family addiction module antidote protein	Antitoxin
SLIT_RS10965	2223785..2224063	-	279	WP_013030320.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
SLIT_RS10970	2224257..2225675	+	1419	WP_013030321.1	PAS domain S-box protein	-
SLIT_RS10975	2225838..2226167	+	330	WP_013030322.1	DUF2007 domain-containing protein	-
SLIT_RS10980	2226196..2226588	-	393	WP_013030323.1	S24 family peptidase	-
SLIT_RS15320	2226987..2228363	+	1377	WP_013030324.1	adenylate/guanylate cyclase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2218119..2233707	15588

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (8-87)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10639.20 Da
• Isoelectric Point: 7.3169

>T24656 WP_013030320.1 NC_013959:c2224063-2223785 [Sideroxydans lithotrophicus ES-1]
MIKSFKHKGLERLFIKGTASGVQADYATRITLMLDAIDVADQVNELDLPGFRLHRLKGVKRNLWSVRVSANWRITFEFEN
GDAYILDLEDYH

Nucleotide

• Length: 279 bp

>T24656 NC_013959:c2224063-2223785 [Sideroxydans lithotrophicus ES-1]
ATGATCAAGAGTTTCAAACATAAAGGACTTGAACGCCTGTTTATCAAAGGCACTGCCAGCGGCGTACAAGCTGACTACGC
CACACGTATTACCCTGATGCTCGACGCAATTGATGTTGCGGATCAGGTGAACGAATTGGACTTGCCCGGCTTCCGGCTGC
ATCGCCTGAAGGGCGTCAAGCGCAACTTGTGGAGCGTGAGAGTGTCGGCTAATTGGCGCATCACATTCGAGTTTGAAAAC
GGCGATGCCTATATACTGGACTTGGAGGATTATCACTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11199.96 Da
• Isoelectric Point: 10.7844

>AT24656 WP_013030319.1 NC_013959:c2223785-2223483 [Sideroxydans lithotrophicus ES-1]
MDTLRNKKRAPTHPGTLLREVVLPELGITQGEFADRLGVSRRTVSEILHGRRPVTPDMAIRLGKLLGNGAGLWLRMQQAV
DVWTLEQEGDYTNIRQLKAA

Nucleotide

• Length: 303 bp

>AT24656 NC_013959:c2223785-2223483 [Sideroxydans lithotrophicus ES-1]
ATGGATACTTTACGAAACAAAAAACGCGCACCCACTCACCCCGGCACATTGCTGCGTGAAGTGGTATTGCCCGAACTGGG
AATCACGCAAGGCGAGTTTGCCGACCGGCTGGGCGTGTCGCGCCGCACCGTGTCCGAAATTCTGCACGGACGCCGTCCGG
TTACGCCGGACATGGCGATCCGCTTGGGCAAATTGCTGGGCAATGGCGCAGGGCTGTGGTTGCGTATGCAACAAGCTGTG
GATGTGTGGACACTGGAACAGGAAGGCGATTACACAAACATCCGGCAACTCAAGGCGGCTTGA

Structures

Toxin

• AlphaFold DB: D5CUN5