Detailed information of TA system TA024651

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 2716181..2716841
• Replicon: chromosome
• Accession: NC_013956
• Organism: Pantoea ananatis LMG 20103

Toxin (Protein)

• Gene name: higB
• Uniprot ID: D4GHT4
• Locus tag: PANA_RS12355
• Protein ID: WP_013026313.1
• Coordinates: 2716488..2716841 (-)
• Length: 118 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: PANA_RS12350
• Protein ID: WP_013026312.1
• Coordinates: 2716181..2716483 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PANA_RS12330	2711199..2711837	+	639	WP_013026308.1	TetR family transcriptional regulator	-
PANA_RS12335	2711911..2713308	+	1398	WP_013026309.1	FAD-binding protein	-
PANA_RS12340	2713310..2714137	+	828	WP_013026310.1	glycosyltransferase family 8 protein	-
PANA_RS12345	2714217..2715338	-	1122	WP_014605487.1	Gfo/Idh/MocA family oxidoreductase	-
PANA_RS12350	2716181..2716483	-	303	WP_013026312.1	XRE family transcriptional regulator	Antitoxin
PANA_RS12355	2716488..2716841	-	354	WP_013026313.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
PANA_RS12360	2717125..2717439	-	315	WP_015699977.1	L-rhamnose mutarotase	-
PANA_RS12365	2717429..2718271	-	843	WP_013026315.1	rhamnulose-1-phosphate aldolase	-
PANA_RS12370	2718282..2719538	-	1257	WP_022623371.1	L-rhamnose isomerase	-
PANA_RS12375	2719522..2721003	-	1482	WP_013026317.1	rhamnulokinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-81)

Sequences

Toxin

Protein

• Length: 118 a.a.
• Molecular weight: 13317.24 Da
• Isoelectric Point: 9.5793

>T24651 WP_013026313.1 NC_013956:c2716841-2716488 [Pantoea ananatis LMG 20103]
VWPIKTTDTFDCWFTSLSDIDRADVLAALLVLREKGPGLSRPYADTLKGSRYSNMKERRIQSRGDPLRAFFAFDPARTGI
VLCAGNKVGNEKRFYDAMLPVADREFTNWLNALKNKE

Nucleotide

• Length: 354 bp

>T24651 NC_013956:c2716841-2716488 [Pantoea ananatis LMG 20103]
GTGTGGCCGATTAAAACAACGGATACGTTTGACTGCTGGTTTACCTCGCTTAGCGATATCGACAGGGCTGACGTGCTTGC
AGCATTGTTGGTCCTGCGGGAGAAGGGGCCGGGTTTGTCCAGACCTTATGCCGATACGCTCAAAGGCTCCCGCTATAGCA
ACATGAAAGAACGACGTATCCAGAGCCGGGGCGATCCTTTACGGGCATTTTTTGCCTTTGACCCGGCCCGAACCGGCATT
GTGCTTTGTGCCGGAAATAAGGTCGGCAATGAAAAACGTTTTTACGACGCGATGCTCCCGGTTGCTGACCGGGAATTCAC
GAACTGGCTTAACGCATTAAAAAATAAGGAGTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11059.77 Da
• Isoelectric Point: 5.1679

>AT24651 WP_013026312.1 NC_013956:c2716483-2716181 [Pantoea ananatis LMG 20103]
MGRTLEQLIADEKNDVVDEAQAMATDILLNIHLAELREKVQKTQVEMAQALGIKQPTVAGMEKPGRDLKLSTLKRYVEAT
GGKLRLDVELPDGSHYGFVL

Nucleotide

• Length: 303 bp

>AT24651 NC_013956:c2716483-2716181 [Pantoea ananatis LMG 20103]
ATGGGCAGAACGCTGGAACAGCTTATTGCGGATGAAAAAAATGACGTCGTGGATGAAGCTCAGGCGATGGCGACGGACAT
CCTGCTCAATATTCACCTTGCCGAACTGCGTGAGAAAGTACAAAAAACGCAGGTAGAAATGGCGCAGGCGCTGGGCATCA
AACAACCTACCGTTGCAGGAATGGAAAAACCGGGGCGCGACCTGAAGCTGTCCACGCTCAAACGCTATGTTGAAGCGACC
GGTGGCAAGCTTCGCCTTGATGTTGAACTGCCGGATGGTTCTCACTACGGTTTTGTGCTTTGA

Structures

Toxin

• AlphaFold DB: D4GHT4