Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 4296305..4296846 Replicon chromosome
Accession NZ_CP097884
Organism Escherichia coli str. K-12 substr. MG1655 strain K-12

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag NAG72_RS20985 Protein ID WP_000615983.1
Coordinates 4296568..4296846 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag NAG72_RS20980 Protein ID WP_000729703.1
Coordinates 4296305..4296565 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NAG72_RS20960 (4291980) 4291980..4292765 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
NAG72_RS20965 (4292737) 4292737..4294449 + 1713 Protein_4078 flagellar biosynthesis protein FlhA -
NAG72_RS20970 (4294673) 4294673..4295170 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
NAG72_RS20975 (4295346) 4295346..4296095 - 750 WP_000056849.1 C40 family peptidase -
NAG72_RS20980 (4296305) 4296305..4296565 + 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
NAG72_RS20985 (4296568) 4296568..4296846 + 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
NAG72_RS20990 (4297002) 4297002..4297742 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
NAG72_RS20995 (4297713) 4297713..4298480 - 768 WP_000333380.1 class II glutamine amidotransferase -
NAG72_RS21000 (4298686) 4298686..4299264 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T246506 WP_000615983.1 NZ_CP097884:4296568-4296846 [Escherichia coli str. K-12 substr. MG1655]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT246506 WP_000729703.1 NZ_CP097884:4296305-4296565 [Escherichia coli str. K-12 substr. MG1655]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References