Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1274409..1275136 Replicon chromosome
Accession NZ_CP097884
Organism Escherichia coli str. K-12 substr. MG1655 strain K-12

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag NAG72_RS06115 Protein ID WP_000550189.1
Coordinates 1274409..1274723 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag NAG72_RS06120 Protein ID WP_000560266.1
Coordinates 1274720..1275136 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NAG72_RS06095 (1270567) 1270567..1271553 - 987 WP_000617698.1 Gfo/Idh/MocA family oxidoreductase -
NAG72_RS06100 (1271632) 1271632..1272324 - 693 WP_000942548.1 vancomycin high temperature exclusion protein -
NAG72_RS06105 (1272401) 1272401..1272904 - 504 WP_001333820.1 M48 family metallopeptidase -
NAG72_RS06110 (1272989) 1272989..1274125 + 1137 WP_000018695.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
NAG72_RS06115 (1274409) 1274409..1274723 + 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
NAG72_RS06120 (1274720) 1274720..1275136 + 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
NAG72_RS06125 (1275181) 1275181..1277199 - 2019 WP_000121433.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
NAG72_RS06130 (1277625) 1277625..1279976 - 2352 WP_000695487.1 alpha-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T246488 WP_000550189.1 NZ_CP097884:1274409-1274723 [Escherichia coli str. K-12 substr. MG1655]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT246488 WP_000560266.1 NZ_CP097884:1274720-1275136 [Escherichia coli str. K-12 substr. MG1655]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References