Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | yafN-yafO (relBE)/YafO-YafN |
| Location | 3663344..3664038 | Replicon | chromosome |
| Accession | NZ_CP097882 | ||
| Organism | Escherichia coli str. K-12 substr. MG1655 strain K-12 | ||
Toxin (Protein)
| Gene name | yafO | Uniprot ID | Q47157 |
| Locus tag | NAG70_RS18000 | Protein ID | WP_001263489.1 |
| Coordinates | 3663344..3663742 (-) | Length | 133 a.a. |
Antitoxin (Protein)
| Gene name | yafN | Uniprot ID | S1QAE3 |
| Locus tag | NAG70_RS18005 | Protein ID | WP_000554758.1 |
| Coordinates | 3663745..3664038 (-) | Length | 98 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| - (3658932) | 3658932..3659012 | - | 81 | NuclAT_12 | - | - |
| - (3658932) | 3658932..3659012 | - | 81 | NuclAT_12 | - | - |
| - (3658932) | 3658932..3659012 | - | 81 | NuclAT_12 | - | - |
| - (3658932) | 3658932..3659012 | - | 81 | NuclAT_12 | - | - |
| NAG70_RS17975 (3659608) | 3659608..3660066 | - | 459 | WP_001291990.1 | xanthine phosphoribosyltransferase | - |
| NAG70_RS17980 (3660327) | 3660327..3661784 | + | 1458 | WP_001292994.1 | cytosol nonspecific dipeptidase | - |
| NAG70_RS17985 (3661841) | 3661841..3662362 | - | 522 | Protein_3514 | peptide chain release factor H | - |
| NAG70_RS17990 (3662358) | 3662358..3662564 | - | 207 | Protein_3515 | RtcB family protein | - |
| NAG70_RS17995 (3662882) | 3662882..3663334 | - | 453 | WP_001059892.1 | GNAT family N-acetyltransferase | - |
| NAG70_RS18000 (3663344) | 3663344..3663742 | - | 399 | WP_001263489.1 | type II toxin-antitoxin system mRNA interferase toxin YafO | Toxin |
| NAG70_RS18005 (3663745) | 3663745..3664038 | - | 294 | WP_000554758.1 | type I toxin-antitoxin system antitoxin YafN | Antitoxin |
| NAG70_RS18010 (3664090) | 3664090..3665145 | - | 1056 | WP_001226164.1 | DNA polymerase IV | - |
| NAG70_RS18015 (3665216) | 3665216..3666001 | - | 786 | WP_000207552.1 | putative lateral flagellar export/assembly protein LafU | - |
| NAG70_RS18020 (3665973) | 3665973..3667685 | + | 1713 | Protein_3521 | flagellar biosynthesis protein FlhA | - |
| NAG70_RS18025 (3667909) | 3667909..3668406 | - | 498 | WP_000006255.1 | REP-associated tyrosine transposase RayT | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 133 a.a. Molecular weight: 15487.84 Da Isoelectric Point: 7.4215
>T246452 WP_001263489.1 NZ_CP097882:c3663742-3663344 [Escherichia coli str. K-12 substr. MG1655]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF
Download Length: 399 bp
Antitoxin
Download Length: 98 a.a. Molecular weight: 11233.76 Da Isoelectric Point: 5.2170
>AT246452 WP_000554758.1 NZ_CP097882:c3664038-3663745 [Escherichia coli str. K-12 substr. MG1655]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE
Download Length: 294 bp
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A090J8B1 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | S1QAE3 |