246374

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 4475120..4475814 Replicon chromosome
Accession NZ_CP097840
Organism Shigella flexneri strain A

Toxin (Protein)


Gene name yafO Uniprot ID Q0T7Q5
Locus tag M9382_RS22530 Protein ID WP_001263491.1
Coordinates 4475416..4475814 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID Q0T7Q4
Locus tag M9382_RS22525 Protein ID WP_000554759.1
Coordinates 4475120..4475413 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M9382_RS22505 (4470759) 4470759..4471256 + 498 WP_000006251.1 REP-associated tyrosine transposase RayT -
M9382_RS22510 (4471473) 4471473..4473185 - 1713 Protein_4381 flagellar biosynthesis protein FlhA -
M9382_RS22515 (4473157) 4473157..4473942 + 786 WP_000207560.1 putative lateral flagellar export/assembly protein LafU -
M9382_RS22520 (4474013) 4474013..4475068 + 1056 WP_001226172.1 DNA polymerase IV -
M9382_RS22525 (4475120) 4475120..4475413 + 294 WP_000554759.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
M9382_RS22530 (4475416) 4475416..4475814 + 399 WP_001263491.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
M9382_RS22535 (4475824) 4475824..4476276 + 453 WP_001059846.1 GNAT family N-acetyltransferase -
M9382_RS22540 (4476522) 4476522..4477565 + 1044 WP_005060486.1 RNA ligase RtcB family protein -
M9382_RS22545 (4477627) 4477627..4478241 + 615 Protein_4388 peptide chain release factor H -
M9382_RS22550 (4478298) 4478298..4479755 - 1458 WP_001293009.1 cytosol nonspecific dipeptidase -
M9382_RS22555 (4480017) 4480017..4480475 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15457.86 Da        Isoelectric Point: 8.0949

>T246374 WP_001263491.1 NZ_CP097840:4475416-4475814 [Shigella flexneri]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11232.82 Da        Isoelectric Point: 7.0153

>AT246374 WP_000554759.1 NZ_CP097840:4475120-4475413 [Shigella flexneri]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
KQYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TPK7

Antitoxin

Source ID Structure
AlphaFold DB A0A4P7TR46

References