Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1596146..1596873 Replicon chromosome
Accession NZ_CP097840
Organism Shigella flexneri strain A

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag M9382_RS07715 Protein ID WP_000550189.1
Coordinates 1596146..1596460 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag M9382_RS07720 Protein ID WP_000560266.1
Coordinates 1596457..1596873 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M9382_RS07695 (1592295) 1592295..1593293 - 999 WP_005050984.1 Gfo/Idh/MocA family oxidoreductase -
M9382_RS07700 (1593372) 1593372..1594064 - 693 WP_000942537.1 vancomycin high temperature exclusion protein -
M9382_RS07705 (1594137) 1594137..1594640 - 504 WP_001295542.1 M48 family metallopeptidase -
M9382_RS07710 (1594725) 1594725..1595861 + 1137 WP_000018658.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
M9382_RS07715 (1596146) 1596146..1596460 + 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
M9382_RS07720 (1596457) 1596457..1596873 + 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
M9382_RS07725 (1596918) 1596918..1598936 - 2019 WP_000121486.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
M9382_RS07730 (1599162) 1599162..1601513 - 2352 WP_000695506.1 alpha-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T246362 WP_000550189.1 NZ_CP097840:1596146-1596460 [Shigella flexneri]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT246362 WP_000560266.1 NZ_CP097840:1596457-1596873 [Shigella flexneri]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References