Detailed information of TA system TA024635

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-couple_hipB
• Location: 663636..664324
• Replicon: chromosome
• Accession: NC_013947
• Organism: Stackebrandtia nassauensis DSM 44728

Toxin (Protein)

• Gene name: HigB2
• Uniprot ID: D3Q6U3
• Locus tag: SNAS_RS03110
• Protein ID: WP_013015913.1
• Coordinates: 663941..664324 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: SNAS_RS03105
• Protein ID: WP_013015912.1
• Coordinates: 663636..663941 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SNAS_RS03085	659369..660580	-	1212	WP_013015908.1	two-component sensor histidine kinase	-
SNAS_RS03090	660721..661581	+	861	WP_013015909.1	CPBP family intramembrane metalloprotease	-
SNAS_RS03095	661578..662228	+	651	WP_013015910.1	response regulator transcription factor	-
SNAS_RS03100	662404..663387	-	984	WP_013015911.1	prolipoprotein diacylglyceryl transferase	-
SNAS_RS03105	663636..663941	-	306	WP_013015912.1	helix-turn-helix domain-containing protein	Antitoxin
SNAS_RS03110	663941..664324	-	384	WP_013015913.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
SNAS_RS03115	664666..665133	-	468	WP_144300382.1	hypothetical protein	-
SNAS_RS03120	665185..666159	-	975	WP_013015915.1	hypothetical protein	-
SNAS_RS33685	666295..666513	-	219	WP_013015916.1	DUF397 domain-containing protein	-
SNAS_RS03125	666520..667416	-	897	WP_169313845.1	helix-turn-helix transcriptional regulator	-
SNAS_RS03130	667548..667895	+	348	WP_013015918.1	hypothetical protein	-
SNAS_RS03135	668193..668435	+	243	WP_144300383.1	hypothetical protein	-
SNAS_RS03140	668492..669013	+	522	WP_144300384.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (20-115)

Antitoxin

• Cro/C1-type helix-turn-helix domain (34-85)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14688.58 Da
• Isoelectric Point: 7.3175

>T24635 WP_013015913.1 NC_013947:c664324-663941 [Stackebrandtia nassauensis DSM 44728]
VAGNYQLRFFKDVIEWIQQLQKEDPDSFEHVIAALERLQEHGPALRRPTVGAIEGSAYRHMRELRARNGSRVSIRLLFAF
DPERRAIFLVAGNKAGQRQWTVWYDKAVKEADARYAAYLDALNAEEE

Nucleotide

• Length: 384 bp

>T24635 NC_013947:c664324-663941 [Stackebrandtia nassauensis DSM 44728]
GTGGCCGGTAACTATCAACTCCGCTTTTTCAAGGACGTCATCGAGTGGATTCAGCAGTTGCAGAAGGAGGACCCTGACAG
CTTTGAGCACGTGATCGCGGCCTTGGAGCGACTCCAAGAGCACGGGCCGGCCCTGCGGAGGCCGACGGTGGGCGCAATCG
AGGGCAGCGCGTATCGACATATGCGAGAACTTCGCGCACGGAACGGCTCGCGTGTCAGTATTCGGCTGTTGTTCGCGTTT
GACCCTGAACGTCGCGCTATCTTTTTGGTCGCAGGCAACAAGGCTGGTCAGCGTCAATGGACAGTTTGGTATGACAAGGC
CGTCAAGGAAGCGGACGCCCGGTACGCCGCGTACCTTGACGCACTAAATGCGGAGGAGGAGTAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 10972.45 Da
• Isoelectric Point: 5.1373

>AT24635 WP_013015912.1 NC_013947:c663941-663636 [Stackebrandtia nassauensis DSM 44728]
MELRDMSELTSGISTEQRARIDAIKQEMIDAERGHELASLRKAQGLTQSQVAEAMGVTQGRISQIESGKTRLDTETMAAY
LHAIGGELTITATVGRLSIQL

Nucleotide

• Length: 306 bp

>AT24635 NC_013947:c663941-663636 [Stackebrandtia nassauensis DSM 44728]
ATGGAACTTCGGGACATGTCCGAACTGACTTCTGGAATTTCCACGGAGCAGCGTGCCCGCATTGACGCGATCAAGCAGGA
AATGATTGACGCTGAACGCGGGCACGAGCTTGCCAGCTTGCGGAAGGCGCAGGGGCTTACCCAATCACAGGTAGCCGAGG
CTATGGGCGTAACTCAGGGACGGATAAGCCAAATCGAAAGCGGCAAAACGCGGTTGGACACCGAGACGATGGCCGCATAT
CTTCACGCCATTGGCGGTGAGCTGACAATCACGGCAACTGTTGGACGTCTGTCCATCCAGCTGTGA

Structures

Toxin

• AlphaFold DB: D3Q6U3