Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 4069905..4070632 Replicon chromosome
Accession NZ_CP097834
Organism Shigella flexneri strain C

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag M9379_RS20570 Protein ID WP_000550189.1
Coordinates 4069905..4070219 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag M9379_RS20575 Protein ID WP_000560266.1
Coordinates 4070216..4070632 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M9379_RS20550 (4066054) 4066054..4067052 - 999 WP_005050984.1 Gfo/Idh/MocA family oxidoreductase -
M9379_RS20555 (4067131) 4067131..4067823 - 693 WP_000942537.1 vancomycin high temperature exclusion protein -
M9379_RS20560 (4067896) 4067896..4068399 - 504 WP_001295542.1 M48 family metallopeptidase -
M9379_RS20565 (4068484) 4068484..4069620 + 1137 WP_000018658.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
M9379_RS20570 (4069905) 4069905..4070219 + 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
M9379_RS20575 (4070216) 4070216..4070632 + 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
M9379_RS20580 (4070677) 4070677..4072695 - 2019 WP_000121486.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
M9379_RS20585 (4072921) 4072921..4075272 - 2352 WP_000695506.1 alpha-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T246332 WP_000550189.1 NZ_CP097834:4069905-4070219 [Shigella flexneri]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT246332 WP_000560266.1 NZ_CP097834:4070216-4070632 [Shigella flexneri]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References