246329

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 2431905..2432599 Replicon chromosome
Accession NZ_CP097834
Organism Shigella flexneri strain C

Toxin (Protein)


Gene name yafO Uniprot ID Q0T7Q5
Locus tag M9379_RS12595 Protein ID WP_001263491.1
Coordinates 2432201..2432599 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID Q0T7Q4
Locus tag M9379_RS12590 Protein ID WP_000554759.1
Coordinates 2431905..2432198 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M9379_RS12570 (2427544) 2427544..2428041 + 498 WP_000006251.1 REP-associated tyrosine transposase RayT -
M9379_RS12575 (2428258) 2428258..2429970 - 1713 Protein_2452 flagellar biosynthesis protein FlhA -
M9379_RS12580 (2429942) 2429942..2430727 + 786 WP_000207560.1 putative lateral flagellar export/assembly protein LafU -
M9379_RS12585 (2430798) 2430798..2431853 + 1056 WP_001226172.1 DNA polymerase IV -
M9379_RS12590 (2431905) 2431905..2432198 + 294 WP_000554759.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
M9379_RS12595 (2432201) 2432201..2432599 + 399 WP_001263491.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
M9379_RS12600 (2432609) 2432609..2433061 + 453 WP_001059846.1 GNAT family N-acetyltransferase -
M9379_RS12605 (2433307) 2433307..2434350 + 1044 WP_005060486.1 RNA ligase RtcB family protein -
M9379_RS12610 (2434412) 2434412..2435026 + 615 Protein_2459 peptide chain release factor H -
M9379_RS12615 (2435083) 2435083..2436540 - 1458 WP_001293009.1 cytosol nonspecific dipeptidase -
M9379_RS12620 (2436802) 2436802..2437260 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - gtrB / gtrA 2424985..2472773 47788


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15457.86 Da        Isoelectric Point: 8.0949

>T246329 WP_001263491.1 NZ_CP097834:2432201-2432599 [Shigella flexneri]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11232.82 Da        Isoelectric Point: 7.0153

>AT246329 WP_000554759.1 NZ_CP097834:2431905-2432198 [Shigella flexneri]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
KQYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TPK7

Antitoxin

Source ID Structure
AlphaFold DB A0A4P7TR46

References