Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 4475936..4476630 Replicon chromosome
Accession NZ_CP097831
Organism Shigella flexneri strain D

Toxin (Protein)


Gene name yafO Uniprot ID Q0T7Q5
Locus tag M9381_RS22540 Protein ID WP_001263491.1
Coordinates 4476232..4476630 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID Q0T7Q4
Locus tag M9381_RS22535 Protein ID WP_000554759.1
Coordinates 4475936..4476229 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M9381_RS22515 (4471575) 4471575..4472072 + 498 WP_000006251.1 REP-associated tyrosine transposase RayT -
M9381_RS22520 (4472289) 4472289..4474001 - 1713 Protein_4383 flagellar biosynthesis protein FlhA -
M9381_RS22525 (4473973) 4473973..4474758 + 786 WP_000207560.1 putative lateral flagellar export/assembly protein LafU -
M9381_RS22530 (4474829) 4474829..4475884 + 1056 WP_001226172.1 DNA polymerase IV -
M9381_RS22535 (4475936) 4475936..4476229 + 294 WP_000554759.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
M9381_RS22540 (4476232) 4476232..4476630 + 399 WP_001263491.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
M9381_RS22545 (4476640) 4476640..4477092 + 453 WP_001059846.1 GNAT family N-acetyltransferase -
M9381_RS22550 (4477338) 4477338..4478381 + 1044 WP_005060486.1 RNA ligase RtcB family protein -
M9381_RS22555 (4478443) 4478443..4479057 + 615 Protein_4390 peptide chain release factor H -
M9381_RS22560 (4479114) 4479114..4480571 - 1458 WP_001293009.1 cytosol nonspecific dipeptidase -
M9381_RS22565 (4480833) 4480833..4481291 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15457.86 Da        Isoelectric Point: 8.0949

>T246313 WP_001263491.1 NZ_CP097831:4476232-4476630 [Shigella flexneri]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11232.82 Da        Isoelectric Point: 7.0153

>AT246313 WP_000554759.1 NZ_CP097831:4475936-4476229 [Shigella flexneri]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
KQYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TPK7

Antitoxin

Source ID Structure
AlphaFold DB A0A4P7TR46

References