Detailed information of TA system TA024631

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 613223..613819
• Replicon: chromosome
• Accession: NC_013943
• Organism: Denitrovibrio acetiphilus DSM 12809

Toxin (Protein)

• Gene name: higB
• Uniprot ID: D4H479
• Locus tag: DACET_RS02990
• Protein ID: WP_013009934.1
• Coordinates: 613223..613504 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: DACET_RS02995
• Protein ID: WP_041230094.1
• Coordinates: 613535..613819 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DACET_RS02980	610225..611874	-	1650	WP_013009932.1	metal-dependent hydrolase	-
DACET_RS02985	611871..613175	-	1305	WP_013009933.1	TraM recognition domain-containing protein	-
DACET_RS02990	613223..613504	+	282	WP_013009934.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
DACET_RS02995	613535..613819	+	285	WP_041230094.1	HigA family addiction module antidote protein	Antitoxin
DACET_RS03000	613828..614511	-	684	WP_013009936.1	hypothetical protein	-
DACET_RS03005	614502..614696	-	195	WP_013009937.1	hypothetical protein	-
DACET_RS03010	614763..615104	-	342	WP_013009938.1	single-stranded DNA-binding protein	-
DACET_RS03015	615244..617292	+	2049	WP_013009939.1	putative Ig domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	550061..690632	140571

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (9-66)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10987.81 Da
• Isoelectric Point: 8.8946

>T24631 WP_013009934.1 NC_013943:613223-613504 [Denitrovibrio acetiphilus DSM 12809]
MIINFADKETEKLFLTGSNRKLLQSIIKRACQMLDILDKTIKVEYPMSPPSYRLHSLSGDLQGFWSISINMQWRIIFRFK
DVDAYDVKITDYH

Nucleotide

• Length: 282 bp

>T24631 NC_013943:613223-613504 [Denitrovibrio acetiphilus DSM 12809]
ATGATAATTAACTTCGCTGATAAAGAGACCGAAAAACTGTTTTTGACTGGCTCCAACAGAAAGCTCCTTCAGAGCATTAT
CAAGAGAGCATGTCAAATGCTTGATATTCTTGATAAGACTATTAAGGTGGAATACCCGATGTCGCCACCCAGTTACAGGC
TCCACAGCCTCAGCGGAGACCTTCAGGGTTTCTGGAGCATCAGCATAAACATGCAATGGCGTATCATCTTCCGTTTTAAA
GATGTAGATGCCTACGATGTTAAAATTACAGACTACCATTAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10630.29 Da
• Isoelectric Point: 8.9140

>AT24631 WP_041230094.1 NC_013943:613535-613819 [Denitrovibrio acetiphilus DSM 12809]
ITRELSHPGEILKDIYIADTPGLTQQKLAYALGVSFRTINQICNKHRGVTPEVAVRLAKYFNTSPELWLNLQMSYDLQKA
ERTVDVSNIKPMAV

Nucleotide

• Length: 285 bp

>AT24631 NC_013943:613535-613819 [Denitrovibrio acetiphilus DSM 12809]
ATAACAAGAGAATTGAGCCACCCCGGGGAAATACTTAAAGATATTTATATAGCCGACACCCCCGGACTCACACAGCAGAA
GCTTGCTTACGCTCTCGGTGTATCTTTTCGTACTATTAATCAGATTTGTAATAAGCACAGAGGTGTGACACCAGAGGTTG
CTGTCAGGCTGGCAAAATATTTTAACACTAGCCCAGAACTCTGGCTTAACCTGCAGATGTCCTACGATCTTCAGAAAGCA
GAGCGCACAGTTGATGTTAGCAATATAAAACCTATGGCGGTGTGA

Structures

Toxin

• AlphaFold DB: D4H479