246283

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 2995845..2996689 Replicon chromosome
Accession NZ_CP097828
Organism Shigella flexneri strain E

Toxin (Protein)


Gene name itaT Uniprot ID A0A4P7TPN5
Locus tag M9383_RS14805 Protein ID WP_005060796.1
Coordinates 2995845..2996243 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID A5H0K0
Locus tag M9383_RS14815 Protein ID WP_005053053.1
Coordinates 2996378..2996689 (-) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M9383_RS14790 (2991013) 2991013..2992743 - 1731 WP_000645011.1 hypothetical protein -
M9383_RS14795 (2992796) 2992796..2994949 - 2154 WP_005060811.1 SEL1-like repeat protein -
M9383_RS14800 (2995100) 2995100..2995797 + 698 WP_232046043.1 IS1 family transposase -
M9383_RS14805 (2995845) 2995845..2996243 - 399 WP_005060796.1 GNAT family N-acetyltransferase Toxin
M9383_RS14810 (2996236) 2996236..2996394 - 159 WP_005053055.1 hypothetical protein -
M9383_RS14815 (2996378) 2996378..2996689 - 312 WP_005053053.1 DUF1778 domain-containing protein Antitoxin
M9383_RS14820 (2996874) 2996874..2997764 - 891 WP_000971356.1 heme o synthase -
M9383_RS14825 (2997776) 2997776..2998105 - 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
M9383_RS14830 (2998105) 2998105..2998719 - 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
M9383_RS14835 (2998709) 2998709..3000700 - 1992 Protein_2877 cytochrome o ubiquinol oxidase subunit I -
M9383_RS14840 (3000722) 3000722..3001219 - 498 Protein_2878 COX aromatic rich motif-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 2995294..2995797 503


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(52-112)

Antitoxin

Vibrio phage ICP1, Orf50

(15-97)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14335.69 Da        Isoelectric Point: 9.1674

>T246283 WP_005060796.1 NZ_CP097828:c2996243-2995845 [Shigella flexneri]
VRNSDCAAKALIDRQSGELIGICTFTAYSLERQRVSGVLQGSQPSEIGVVRLVMLGGARKYQKRGFGQDLLCDFFEHVKK
IHPALPIKGVYLDADPAAINFYARLGFVQLSATPNAFGAVPMFLAIQHILAA

Download         Length: 399 bp

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11394.07 Da        Isoelectric Point: 10.3388

>AT246283 WP_005053053.1 NZ_CP097828:c2996689-2996378 [Shigella flexneri]
MPGKTAALYDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TPN5

Antitoxin

Source ID Structure
AlphaFold DB A5H0K0

References