Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 2913999..2914693 Replicon chromosome
Accession NZ_CP097828
Organism Shigella flexneri strain E

Toxin (Protein)


Gene name yafO Uniprot ID Q0T7Q5
Locus tag M9383_RS14380 Protein ID WP_001263491.1
Coordinates 2913999..2914397 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID Q0T7Q4
Locus tag M9383_RS14385 Protein ID WP_000554759.1
Coordinates 2914400..2914693 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M9383_RS14355 (2909338) 2909338..2909796 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
M9383_RS14360 (2910058) 2910058..2911515 + 1458 WP_001293009.1 cytosol nonspecific dipeptidase -
M9383_RS14365 (2911572) 2911572..2912132 - 561 Protein_2783 peptide chain release factor H -
M9383_RS14370 (2912248) 2912248..2913291 - 1044 WP_005060486.1 RNA ligase RtcB family protein -
M9383_RS14375 (2913537) 2913537..2913989 - 453 WP_001059846.1 GNAT family N-acetyltransferase -
M9383_RS14380 (2913999) 2913999..2914397 - 399 WP_001263491.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
M9383_RS14385 (2914400) 2914400..2914693 - 294 WP_000554759.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
M9383_RS14390 (2914745) 2914745..2915800 - 1056 WP_001226172.1 DNA polymerase IV -
M9383_RS14395 (2915871) 2915871..2916656 - 786 WP_000207560.1 putative lateral flagellar export/assembly protein LafU -
M9383_RS14400 (2916628) 2916628..2918340 + 1713 Protein_2790 flagellar biosynthesis protein FlhA -
M9383_RS14405 (2918557) 2918557..2919054 - 498 WP_000006251.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - gtrB / gmhA/lpcA 2873825..2962866 89041


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15457.86 Da        Isoelectric Point: 8.0949

>T246282 WP_001263491.1 NZ_CP097828:c2914397-2913999 [Shigella flexneri]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11232.82 Da        Isoelectric Point: 7.0153

>AT246282 WP_000554759.1 NZ_CP097828:c2914693-2914400 [Shigella flexneri]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
KQYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TPK7

Antitoxin

Source ID Structure
AlphaFold DB A0A4P7TR46

References