Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1275140..1275867 Replicon chromosome
Accession NZ_CP097828
Organism Shigella flexneri strain E

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag M9383_RS06415 Protein ID WP_000550189.1
Coordinates 1275553..1275867 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag M9383_RS06410 Protein ID WP_000560266.1
Coordinates 1275140..1275556 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M9383_RS06400 (1270500) 1270500..1272851 + 2352 WP_000695506.1 alpha-glucosidase -
M9383_RS06405 (1273077) 1273077..1275095 + 2019 WP_000121486.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
M9383_RS06410 (1275140) 1275140..1275556 - 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
M9383_RS06415 (1275553) 1275553..1275867 - 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
M9383_RS06420 (1276152) 1276152..1277288 - 1137 WP_000018658.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
M9383_RS06425 (1277373) 1277373..1277876 + 504 WP_001295542.1 M48 family metallopeptidase -
M9383_RS06430 (1277949) 1277949..1278641 + 693 WP_000942537.1 vancomycin high temperature exclusion protein -
M9383_RS06435 (1278720) 1278720..1279718 + 999 WP_005050984.1 Gfo/Idh/MocA family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T246279 WP_000550189.1 NZ_CP097828:c1275867-1275553 [Shigella flexneri]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT246279 WP_000560266.1 NZ_CP097828:c1275556-1275140 [Shigella flexneri]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References