Detailed information of TA system TA246223

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 28594..28858
• Replicon: plasmid pMS1050-2
• Accession: NZ_CP097728
• Organism: Escherichia coli strain MS1050

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: U9Y6M3
• Locus tag: M9O78_RS25000
• Protein ID: WP_001331364.1
• Coordinates: 28594..28746 (-)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	28801..28858 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M9O78_RS24975 (23872)	23872..26034	+	2163	WP_000698351.1	DotA/TraY family protein	-
M9O78_RS24980 (26099)	26099..26761	+	663	WP_000653334.1	plasmid IncI1-type surface exclusion protein ExcA	-
M9O78_RS24985 (26833)	26833..27042	-	210	WP_000062603.1	HEAT repeat domain-containing protein	-
M9O78_RS24990 (27434)	27434..27610	+	177	WP_001054904.1	hypothetical protein	-
M9O78_RS24995 (27675)	27675..27971	-	297	WP_001275298.1	DinQ-like type I toxin DqlB	-
M9O78_RS25000 (28594)	28594..28746	-	153	WP_001331364.1	Hok/Gef family protein	Toxin
- (28801)	28801..28858	+	58	NuclAT_0	-	Antitoxin
- (28801)	28801..28858	+	58	NuclAT_0	-	Antitoxin
- (28801)	28801..28858	+	58	NuclAT_0	-	Antitoxin
- (28801)	28801..28858	+	58	NuclAT_0	-	Antitoxin
M9O78_RS25005 (29038)	29038..30246	+	1209	WP_000121274.1	IncI1-type conjugal transfer protein TrbA	-
M9O78_RS25010 (30265)	30265..31335	+	1071	WP_000151583.1	IncI1-type conjugal transfer protein TrbB	-
M9O78_RS25015 (31328)	31328..33619	+	2292	WP_001289276.1	F-type conjugative transfer protein TrbC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	ant(3'')-Ia / aac(3)-VIa / qacE / sul1 / tet(A)	-	1..117915	117915

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5775.13 Da
• Isoelectric Point: 8.7948

>T246223 WP_001331364.1 NZ_CP097728:c28746-28594 [Escherichia coli]
MPQRTFLMMLIVICVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

Antitoxin

Nucleotide

• Length: 58 bp

>AT246223 NZ_CP097728:28801-28858 [Escherichia coli]
GCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: A0A5W9M382

Antitoxin