Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2554724..2555286 Replicon chromosome
Accession NZ_CP097726
Organism Escherichia coli strain MS1050

Toxin (Protein)


Gene name higB Uniprot ID L4J948
Locus tag M9O78_RS12165 Protein ID WP_000605676.1
Coordinates 2554724..2555002 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag M9O78_RS12170 Protein ID WP_000781370.1
Coordinates 2555002..2555286 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M9O78_RS12140 (2550310) 2550310..2550741 - 432 WP_000152305.1 peroxiredoxin OsmC -
M9O78_RS12145 (2551086) 2551086..2551301 + 216 WP_000495766.1 biofilm-dependent modulation protein -
M9O78_RS12150 (2551403) 2551403..2551540 + 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
M9O78_RS12155 (2551697) 2551697..2553394 + 1698 WP_024241319.1 malate dehydrogenase -
M9O78_RS12160 (2553528) 2553528..2554538 + 1011 WP_000642407.1 alcohol dehydrogenase AdhP -
M9O78_RS12165 (2554724) 2554724..2555002 + 279 WP_000605676.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
M9O78_RS12170 (2555002) 2555002..2555286 + 285 WP_000781370.1 HigA family addiction module antitoxin Antitoxin
M9O78_RS12175 (2555514) 2555514..2556167 - 654 WP_000045646.1 formate dehydrogenase-N subunit gamma -
M9O78_RS12180 (2556160) 2556160..2557044 - 885 WP_001240565.1 formate dehydrogenase N subunit beta -
M9O78_RS12185 (2557057) 2557057..2560104 - 3048 WP_012602782.1 formate dehydrogenase-N subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10521.10 Da        Isoelectric Point: 7.3562

>T246204 WP_000605676.1 NZ_CP097726:2554724-2555002 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAASCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT246204 WP_000781370.1 NZ_CP097726:2555002-2555286 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G6T1

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References