Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2541381..2541883 Replicon chromosome
Accession NC_013941
Organism Escherichia coli O55:H7 str. CB9615

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag G2583_RS13050 Protein ID WP_000767829.1
Coordinates 2541381..2541635 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag G2583_RS13055 Protein ID WP_001259255.1
Coordinates 2541632..2541883 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G2583_RS13025 2536396..2536623 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
G2583_RS13030 2536637..2537695 - 1059 WP_000492328.1 transport protein YeeE -
G2583_RS13035 2537874..2539232 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
G2583_RS31195 2539222..2539284 - 63 WP_010723108.1 membrane protein YoeI -
G2583_RS13040 2539499..2540428 - 930 WP_000803334.1 LysR family transcriptional regulator -
G2583_RS13045 2540474..2541298 - 825 WP_000754737.1 SDR family oxidoreductase -
G2583_RS13050 2541381..2541635 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
G2583_RS13055 2541632..2541883 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
G2583_RS28760 2542167..2542217 + 51 WP_013009236.1 his operon leader peptide -
G2583_RS13060 2542363..2543262 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
G2583_RS13065 2543268..2544572 + 1305 WP_001419115.1 histidinol dehydrogenase -
G2583_RS13070 2544569..2545639 + 1071 WP_000108999.1 histidinol-phosphate transaminase -
G2583_RS13075 2545639..2546706 + 1068 WP_000080093.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T24618 WP_000767829.1 NC_013941:c2541635-2541381 [Escherichia coli O55:H7 str. CB9615]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T24618 NC_013941:c2541635-2541381 [Escherichia coli O55:H7 str. CB9615]
GTGAAACTAATCTGGTCTGAGGAGTCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT24618 WP_001259255.1 NC_013941:c2541883-2541632 [Escherichia coli O55:H7 str. CB9615]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT24618 NC_013941:c2541883-2541632 [Escherichia coli O55:H7 str. CB9615]
ATGCGTACAATTAGCTACAGCGAAGCGCGCCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCTCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References