Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 3703672..3704351 Replicon chromosome
Accession NZ_CP097719
Organism Escherichia coli strain MS1679

Toxin (Protein)


Gene name higB Uniprot ID S1PK60
Locus tag M9O74_RS18320 Protein ID WP_000057523.1
Coordinates 3704049..3704351 (-) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag M9O74_RS18315 Protein ID WP_000806442.1
Coordinates 3703672..3704013 (-) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M9O74_RS18305 (3699915) 3699915..3700847 - 933 WP_000883024.1 glutaminase A -
M9O74_RS18310 (3701110) 3701110..3703614 + 2505 WP_000083999.1 copper-exporting P-type ATPase CopA -
M9O74_RS18315 (3703672) 3703672..3704013 - 342 WP_000806442.1 HigA family addiction module antitoxin Antitoxin
M9O74_RS18320 (3704049) 3704049..3704351 - 303 WP_000057523.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
M9O74_RS18325 (3704484) 3704484..3705278 + 795 WP_000365161.1 TraB/GumN family protein -
M9O74_RS18330 (3705482) 3705482..3705961 + 480 WP_000186638.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
M9O74_RS18335 (3705998) 3705998..3707650 - 1653 Protein_3595 bifunctional UDP-sugar hydrolase/5'-nucleotidase -
M9O74_RS18340 (3707868) 3707868..3709088 + 1221 WP_001251634.1 fosmidomycin MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3695558..3704351 8793


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11809.44 Da        Isoelectric Point: 10.3980

>T246135 WP_000057523.1 NZ_CP097719:c3704351-3704049 [Escherichia coli]
MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT246135 WP_000806442.1 NZ_CP097719:c3704013-3703672 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PK60

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References