Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3985073..3985767 Replicon chromosome
Accession NZ_CP097716
Organism Escherichia coli strain MS1718

Toxin (Protein)


Gene name yafO Uniprot ID Q0T7Q5
Locus tag M9O77_RS19070 Protein ID WP_001263491.1
Coordinates 3985073..3985471 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID L4JHF0
Locus tag M9O77_RS19075 Protein ID WP_000554755.1
Coordinates 3985474..3985767 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M9O77_RS19040 (3980335) 3980335..3981792 + 1458 WP_022645227.1 cytosol nonspecific dipeptidase -
M9O77_RS19045 (3981801) 3981801..3982082 + 282 WP_022645226.1 hypothetical protein -
M9O77_RS19050 (3982099) 3982099..3982608 - 510 WP_001361775.1 metal-dependent hydrolase -
M9O77_RS19055 (3982670) 3982670..3983284 - 615 WP_022645225.1 peptide chain release factor H -
M9O77_RS19060 (3983281) 3983281..3984420 - 1140 WP_022645224.1 RNA ligase RtcB family protein -
M9O77_RS19065 (3984611) 3984611..3985063 - 453 WP_023909048.1 GNAT family N-acetyltransferase -
M9O77_RS19070 (3985073) 3985073..3985471 - 399 WP_001263491.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
M9O77_RS19075 (3985474) 3985474..3985767 - 294 WP_000554755.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
M9O77_RS19080 (3985819) 3985819..3986874 - 1056 WP_022645222.1 DNA polymerase IV -
M9O77_RS19085 (3986945) 3986945..3987730 - 786 WP_000207551.1 putative lateral flagellar export/assembly protein LafU -
M9O77_RS19090 (3987702) 3987702..3989414 + 1713 Protein_3743 flagellar biosynthesis protein FlhA -
M9O77_RS19095 (3989512) 3989512..3990285 - 774 WP_022645219.1 C40 family peptidase -
M9O77_RS19100 (3990471) 3990471..3990731 + 261 WP_000729708.1 type II toxin-antitoxin system antitoxin DinJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15457.86 Da        Isoelectric Point: 8.0949

>T246100 WP_001263491.1 NZ_CP097716:c3985471-3985073 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11235.78 Da        Isoelectric Point: 5.2170

>AT246100 WP_000554755.1 NZ_CP097716:c3985767-3985474 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TPK7

Antitoxin

Source ID Structure
AlphaFold DB A0A829G9H5

References