246040

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 2488666..2489441 Replicon chromosome
Accession NZ_CP097709
Organism Pseudomonas aeruginosa strain PA-1

Toxin (Protein)


Gene name yhaV Uniprot ID V6ADY6
Locus tag M8341_RS11900 Protein ID WP_009518525.1
Coordinates 2488983..2489441 (+) Length 153 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID V6AEP7
Locus tag M8341_RS11895 Protein ID WP_023098543.1
Coordinates 2488666..2488983 (+) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M8341_RS11875 (M8341_11870) 2483836..2485224 - 1389 Protein_2346 efflux RND transporter permease subunit -
M8341_RS11880 (M8341_11875) 2485361..2486272 + 912 WP_023098540.1 NAD(P)H-binding protein -
M8341_RS11885 (M8341_11880) 2486421..2486567 + 147 WP_023098541.1 MerR family DNA-binding transcriptional regulator -
M8341_RS11890 (M8341_11885) 2486549..2488366 - 1818 WP_023098542.1 MobH family relaxase -
M8341_RS11895 (M8341_11890) 2488666..2488983 + 318 WP_023098543.1 type II toxin-antitoxin system PrlF family antitoxin Antitoxin
M8341_RS11900 (M8341_11895) 2488983..2489441 + 459 WP_009518525.1 type II toxin-antitoxin system YhaV family toxin Toxin
M8341_RS11905 (M8341_11900) 2489468..2489845 + 378 WP_009518524.1 DUF3742 family protein -
M8341_RS11910 (M8341_11905) 2489861..2491378 - 1518 WP_009518523.1 conjugal transfer protein TraG N-terminal domain-containing protein -
M8341_RS11915 (M8341_11910) 2491393..2491752 - 360 WP_009518522.1 hypothetical protein -
M8341_RS11920 (M8341_11915) 2491749..2493143 - 1395 WP_009518521.1 integrating conjugative element protein -
M8341_RS11925 (M8341_11920) 2493153..2494100 - 948 WP_009518520.1 TIGR03756 family integrating conjugative element protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - - 2414202..2551252 137050


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(8-144)

Antitoxin

PrlF antitoxin

(8-100)


Sequences

Toxin        


Download         Length: 153 a.a.        Molecular weight: 17660.02 Da        Isoelectric Point: 10.1848

>T246040 WP_009518525.1 NZ_CP097709:2488983-2489441 [Pseudomonas aeruginosa]
MQRHGWTLLFHDCVIEQLQKLHAAARRAQENDPAGFESNANVKLFRALSQLMLDVVPGDPARDEYRQGNTLGPAHRHWRR
AKIGRRFRLFFRYDSKAKVIVYAWVNDEQTLRSSGSKSDPYVVFEKMLGRGNPPDDWHALIQASKQDWSKLE

Download         Length: 459 bp

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11170.72 Da        Isoelectric Point: 4.7182

>AT246040 WP_023098543.1 NZ_CP097709:2488666-2488983 [Pseudomonas aeruginosa]
MPAIHEVATLTSKGQITLPKPIRQALGVDAGGKLAFDLRGSEVVVTRVDAEHEDPAIGAFLSLLARDIEAGRNVRGLPED
LARAMLEHAGHGVNLDEEIDGKVEL

Download         Length: 318 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V6ADY6

Antitoxin

Source ID Structure
AlphaFold DB V6AEP7

References