Detailed information of TA system TA024604

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 1733804..1734175
• Replicon: chromosome
• Accession: NC_013941
• Organism: Escherichia coli O55:H7 str. CB9615

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: A0A0D6ZRD3
• Locus tag: G2583_RS08660
• Protein ID: WP_001443846.1
• Coordinates: 1734026..1734175 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	1733804..1733982 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G2583_RS31550	1729556..1729726	+	171	WP_001625136.1	hypothetical protein	-
G2583_RS08635	1729759..1731132	+	1374	WP_000123746.1	ATP-dependent RNA helicase DbpA	-
G2583_RS08640	1731261..1732196	-	936	WP_000662472.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
G2583_RS28455	1732248..1733483	-	1236	WP_000040839.1	site-specific integrase	-
G2583_RS08650	1733485..1733700	-	216	WP_000079604.1	excisionase XisR	-
G2583_RS08655	1733800..1733988	-	189	WP_001302840.1	DUF1187 family protein	-
-	1733804..1733982	+	179	NuclAT_0	-	Antitoxin
-	1733804..1733982	+	179	NuclAT_0	-	Antitoxin
-	1733804..1733982	+	179	NuclAT_0	-	Antitoxin
-	1733804..1733982	+	179	NuclAT_0	-	Antitoxin
G2583_RS08660	1734026..1734175	-	150	WP_001443846.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
G2583_RS08665	1734231..1735040	-	810	WP_000166313.1	recombination protein RecT	-
G2583_RS08670	1735033..1737705	-	2673	WP_000102213.1	exodeoxyribonuclease VIII	-
G2583_RS08675	1737805..1738080	-	276	WP_001452559.1	hypothetical protein	-
G2583_RS08680	1738155..1738331	-	177	WP_000245528.1	hypothetical protein	-
G2583_RS08685	1738325..1738546	-	222	WP_000560227.1	cell division protein FtsZ	-
G2583_RS08690	1738955..1739107	-	153	WP_000379547.1	DUF1391 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1732248..1774195	41947

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-48)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5383.12 Da
• Isoelectric Point: 8.3398

>T24604 WP_001443846.1 NC_013941:c1734175-1734026 [Escherichia coli O55:H7 str. CB9615]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALER

Nucleotide

• Length: 150 bp

>T24604 NC_013941:c1734175-1734026 [Escherichia coli O55:H7 str. CB9615]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAACGATGA

Antitoxin

Nucleotide

• Length: 179 bp

>AT24604 NC_013941:1733804-1733982 [Escherichia coli O55:H7 str. CB9615]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A0D6ZRD3

Antitoxin