Detailed information of TA system TA024601

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 292453..293147
• Replicon: chromosome
• Accession: NC_013941
• Organism: Escherichia coli O55:H7 str. CB9615

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: C3TNX7
• Locus tag: G2583_RS01365
• Protein ID: WP_001263495.1
• Coordinates: 292749..293147 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: G2583_RS01360
• Protein ID: WP_000554757.1
• Coordinates: 292453..292746 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G2583_RS30765	288076..288573	+	498	Protein_250	REP-associated tyrosine transposase RayT	-
G2583_RS01345	288806..290518	-	1713	Protein_251	flagellar biosynthesis protein FlhA	-
G2583_RS01350	290490..291275	+	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
G2583_RS01355	291346..292401	+	1056	WP_001226186.1	DNA polymerase IV	-
G2583_RS01360	292453..292746	+	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
G2583_RS01365	292749..293147	+	399	WP_001263495.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
G2583_RS01370	293157..293609	+	453	WP_001059871.1	GNAT family N-acetyltransferase	-
G2583_RS01375	293928..294131	+	204	Protein_257	RNA ligase RtcB family protein	-
G2583_RS01380	294130..294651	+	522	Protein_258	peptide chain release factor H	-
G2583_RS01385	294708..296165	-	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
G2583_RS01390	296426..296884	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
-	297480..297560	+	81	NuclAT_11	-	-
-	297480..297560	+	81	NuclAT_11	-	-
-	297480..297560	+	81	NuclAT_11	-	-
-	297480..297560	+	81	NuclAT_11	-	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15473.86 Da
• Isoelectric Point: 8.0949

>T24601 WP_001263495.1 NC_013941:292749-293147 [Escherichia coli O55:H7 str. CB9615]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T24601 NC_013941:292749-293147 [Escherichia coli O55:H7 str. CB9615]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAGCTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACTTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAATCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT24601 WP_000554757.1 NC_013941:292453-292746 [Escherichia coli O55:H7 str. CB9615]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT24601 NC_013941:292453-292746 [Escherichia coli O55:H7 str. CB9615]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAATTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Antitoxin

• AlphaFold DB: S1FJN6