Detailed information of TA system TA024600

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DinJ-YafQ (relBE)/YafQ-RelB
• Location: 286400..286941
• Replicon: chromosome
• Accession: NC_013941
• Organism: Escherichia coli O55:H7 str. CB9615

Toxin (Protein)

• Gene name: yafQ
• Uniprot ID: S1FHQ9
• Locus tag: G2583_RS01325
• Protein ID: WP_000615979.1
• Coordinates: 286400..286678 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: C3TP07
• Locus tag: G2583_RS01330
• Protein ID: WP_000729705.1
• Coordinates: 286681..286941 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G2583_RS01310	284083..284661	+	579	WP_000284050.1	D-sedoheptulose 7-phosphate isomerase	-
G2583_RS01315	284766..285533	+	768	WP_001301698.1	class II glutamine amidotransferase	-
G2583_RS01320	285504..286244	-	741	WP_001225679.1	murein L,D-transpeptidase	-
G2583_RS01325	286400..286678	-	279	WP_000615979.1	type II toxin-antitoxin system mRNA interferase toxin YafQ	Toxin
G2583_RS01330	286681..286941	-	261	WP_000729705.1	type II toxin-antitoxin system antitoxin DinJ	Antitoxin
G2583_RS01335	287127..287900	+	774	WP_000543891.1	C40 family peptidase	-
G2583_RS30765	288076..288573	+	498	Protein_250	REP-associated tyrosine transposase RayT	-
G2583_RS01345	288806..290518	-	1713	Protein_251	flagellar biosynthesis protein FlhA	-
G2583_RS01350	290490..291275	+	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (1-91)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-85)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10830.62 Da
• Isoelectric Point: 10.0702

>T24600 WP_000615979.1 NC_013941:c286678-286400 [Escherichia coli O55:H7 str. CB9615]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Nucleotide

• Length: 279 bp

>T24600 NC_013941:c286678-286400 [Escherichia coli O55:H7 str. CB9615]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9377.74 Da
• Isoelectric Point: 5.0758

>AT24600 WP_000729705.1 NC_013941:c286941-286681 [Escherichia coli O55:H7 str. CB9615]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGV

Nucleotide

• Length: 261 bp

>AT24600 NC_013941:c286941-286681 [Escherichia coli O55:H7 str. CB9615]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAGTTTAA

Structures

Toxin

• AlphaFold DB: S1FHQ9