Detailed information of TA system TA245927

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 148478..148904
• Replicon: plasmid unnamed3
• Accession: NZ_CP097686
• Organism: Klebsiella pneumoniae strain IR12073_1

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: M3X91_RS28640
• Protein ID: WP_001372321.1
• Coordinates: 148779..148904 (+)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	148478..148702 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M3X91_RS28605 (143588)	143588..144043	-	456	Protein_190	hypothetical protein	-
M3X91_RS28610 (144345)	144345..144872	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
M3X91_RS28615 (144930)	144930..145163	+	234	WP_000006003.1	DUF905 family protein	-
M3X91_RS28620 (145224)	145224..147247	+	2024	Protein_193	ParB/RepB/Spo0J family partition protein	-
M3X91_RS28625 (147316)	147316..147750	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
M3X91_RS28630 (147747)	147747..148466	+	720	WP_001276217.1	plasmid SOS inhibition protein A	-
- (148478)	148478..148702	+	225	NuclAT_0	-	Antitoxin
- (148478)	148478..148702	+	225	NuclAT_0	-	Antitoxin
- (148478)	148478..148702	+	225	NuclAT_0	-	Antitoxin
- (148478)	148478..148702	+	225	NuclAT_0	-	Antitoxin
M3X91_RS28635 (148688)	148688..148837	+	150	Protein_196	plasmid maintenance protein Mok	-
M3X91_RS28640 (148779)	148779..148904	+	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
M3X91_RS28645 (149223)	149223..149519	-	297	Protein_198	hypothetical protein	-
M3X91_RS28650 (149819)	149819..150115	+	297	WP_001272251.1	hypothetical protein	-
M3X91_RS28655 (150226)	150226..151047	+	822	WP_001234445.1	DUF932 domain-containing protein	-
M3X91_RS28660 (151344)	151344..151991	-	648	WP_015059008.1	transglycosylase SLT domain-containing protein	-
M3X91_RS28665 (152268)	152268..152651	+	384	WP_000124981.1	conjugal transfer relaxosome DNA-binding protein TraM	-
M3X91_RS28670 (152842)	152842..153527	+	686	Protein_203	PAS domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaKPC-2 / catA2 / blaCTX-M-65	-	1..153836	153836

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T245927 WP_001372321.1 NZ_CP097686:148779-148904 [Klebsiella pneumoniae]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

Antitoxin

Nucleotide

• Length: 225 bp

>AT245927 NZ_CP097686:148478-148702 [Klebsiella pneumoniae]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Antitoxin