Detailed information of TA system TA245835

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 122457..123114
• Replicon: plasmid unnamed4
• Accession: NZ_CP097667
• Organism: Klebsiella pneumoniae strain IR1230_1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U3PDC3
• Locus tag: M3X90_RS29005
• Protein ID: WP_000270043.1
• Coordinates: 122457..122807 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: M3X90_RS29010
• Protein ID: WP_000124640.1
• Coordinates: 122812..123114 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M3X90_RS28960 (M3X90_28960)	117768..117947	+	180	Protein_128	helix-turn-helix domain-containing protein	-
M3X90_RS28965 (M3X90_28965)	118012..118716	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-
M3X90_RS28970 (M3X90_28970)	118931..119428	-	498	WP_000062185.1	hypothetical protein	-
M3X90_RS28975 (M3X90_28975)	119431..119919	-	489	WP_001273096.1	DUF1643 domain-containing protein	-
M3X90_RS28980 (M3X90_28980)	120016..120351	+	336	WP_000683476.1	hypothetical protein	-
M3X90_RS28985 (M3X90_28985)	120366..120836	-	471	WP_001281821.1	hypothetical protein	-
M3X90_RS28990 (M3X90_28990)	120829..121200	-	372	WP_000516916.1	hypothetical protein	-
M3X90_RS28995 (M3X90_28995)	121211..121405	-	195	WP_000343597.1	hypothetical protein	-
M3X90_RS29000 (M3X90_29000)	121746..122294	-	549	WP_001061574.1	transcriptional regulator	-
M3X90_RS29005 (M3X90_29005)	122457..122807	+	351	WP_000270043.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
M3X90_RS29010 (M3X90_29010)	122812..123114	+	303	WP_000124640.1	XRE family transcriptional regulator	Antitoxin
M3X90_RS29015 (M3X90_29015)	123141..123434	-	294	WP_001239997.1	chromosome segregation protein ParM	-
M3X90_RS29020 (M3X90_29020)	123522..123794	-	273	WP_001043047.1	HU family DNA-binding protein	-
M3X90_RS29025 (M3X90_29025)	123852..124379	-	528	WP_001236377.1	thermonuclease family protein	-
M3X90_RS29030 (M3X90_29030)	124610..125467	-	858	WP_001167032.1	hypothetical protein	-
M3X90_RS29035 (M3X90_29035)	125454..125684	-	231	WP_000972663.1	hypothetical protein	-
M3X90_RS29040 (M3X90_29040)	125684..126202	-	519	WP_000210756.1	nitrite reductase	-
M3X90_RS29045 (M3X90_29045)	126199..126645	-	447	WP_000919345.1	Fe3+-siderophore ABC transporter permease	-
M3X90_RS29050 (M3X90_29050)	126645..127004	-	360	WP_000422768.1	hypothetical protein	-
M3X90_RS29055 (M3X90_29055)	127061..127489	-	429	WP_000591074.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sul1 / qacE / dfrA25 / blaCMY-2 / sul2 / aph(3'')-Ib / aph(6)-Id / tet(A) / floR	-	1..153479	153479

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13328.21 Da
• Isoelectric Point: 5.2421

>T245835 WP_000270043.1 NZ_CP097667:122457-122807 [Klebsiella pneumoniae]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE

Nucleotide

• Length: 351 bp

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11006.73 Da
• Isoelectric Point: 7.2036

>AT245835 WP_000124640.1 NZ_CP097667:122812-123114 [Klebsiella pneumoniae]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV

Nucleotide

• Length: 303 bp

Structures

Toxin

• AlphaFold DB: A0A7G8AFB9