Detailed information of TA system TA024580

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1248462..1249036
• Replicon: chromosome
• Accession: NC_013892
• Organism: Xenorhabdus bovienii SS-2004

Toxin (Protein)

• Gene name: higB
• Uniprot ID: D3UXL4
• Locus tag: XBJ1_RS05445
• Protein ID: WP_012987798.1
• Coordinates: 1248755..1249036 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: XBJ1_RS05440
• Protein ID: WP_012987797.1
• Coordinates: 1248462..1248743 (-)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
XBJ1_RS22160	1243710..1244135	+	426	Protein_1089	helix-turn-helix domain-containing protein	-
XBJ1_RS05415	1244484..1245068	+	585	WP_012987792.1	ATP-binding protein	-
XBJ1_RS05420	1245065..1246426	+	1362	WP_012987793.1	replicative DNA helicase	-
XBJ1_RS05425	1246445..1246753	+	309	WP_012987794.1	antitermination protein	-
XBJ1_RS05430	1247054..1247254	+	201	WP_012987795.1	hypothetical protein	-
XBJ1_RS05435	1247354..1248391	+	1038	WP_012987796.1	site-specific DNA-methyltransferase	-
XBJ1_RS05440	1248462..1248743	-	282	WP_012987797.1	HigA family addiction module antidote protein	Antitoxin
XBJ1_RS05445	1248755..1249036	-	282	WP_012987798.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
XBJ1_RS05450	1249192..1249530	+	339	WP_038198466.1	phage holin, lambda family	-
XBJ1_RS05455	1249514..1249948	+	435	WP_012987800.1	lysozyme	-
XBJ1_RS20610	1249945..1250418	+	474	WP_012987801.1	lysis protein	-
XBJ1_RS05465	1250529..1250840	+	312	WP_012987802.1	cupin	-
XBJ1_RS05470	1250970..1251320	+	351	WP_012987803.1	HNH endonuclease	-
XBJ1_RS05475	1251516..1252010	+	495	WP_012987805.1	phage terminase small subunit P27 family	-
XBJ1_RS05480	1252007..1253737	+	1731	WP_012987806.1	terminase large subunit	-
XBJ1_RS21970	1253747..1253923	+	177	WP_012987807.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1137911..1304106	166195

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (3-93)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (4-81)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10436.99 Da
• Isoelectric Point: 10.3207

>T24580 WP_012987798.1 NC_013892:c1249036-1248755 [Xenorhabdus bovienii SS-2004]
MIKSFKHKGLEKFFKTGSTAGIQANHAVKLNIQLTALNTAKKPDDMNAPGWKLPPLKGADLKGHWAVSVNGNWRLTFRFE
GEDAILVNYQDYH

Nucleotide

• Length: 282 bp

>T24580 NC_013892:c1249036-1248755 [Xenorhabdus bovienii SS-2004]
ATGATTAAGAGTTTTAAACATAAAGGGTTAGAAAAATTCTTTAAAACAGGCTCTACAGCAGGCATTCAGGCGAACCATGC
TGTAAAACTGAATATTCAATTAACCGCGTTAAACACCGCCAAAAAACCCGATGACATGAACGCGCCCGGCTGGAAATTAC
CCCCGTTAAAAGGTGCTGATTTAAAAGGCCACTGGGCTGTTTCTGTCAACGGGAACTGGCGGCTGACATTTCGCTTTGAA
GGCGAAGATGCCATTTTGGTTAATTATCAAGATTATCACTGA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10204.71 Da
• Isoelectric Point: 7.3180

>AT24580 WP_012987797.1 NC_013892:c1248743-1248462 [Xenorhabdus bovienii SS-2004]
MSRMHNPAHPGIVLREYLGDISVTEAAKALGVTRVALSRILNGSAGISADMALRLEAALGTSAEMWTSMQSQYELWQASQ
QQRPEIRPIFAHL

Nucleotide

• Length: 282 bp

>AT24580 NC_013892:c1248743-1248462 [Xenorhabdus bovienii SS-2004]
ATGAGCAGAATGCATAATCCCGCCCATCCCGGCATTGTCTTACGCGAATATTTAGGCGACATTTCTGTTACAGAAGCGGC
AAAAGCGCTGGGCGTAACCCGTGTGGCTTTATCCCGTATTTTAAATGGCAGCGCAGGCATATCCGCAGATATGGCACTTC
GTTTAGAAGCGGCATTGGGAACCAGTGCCGAAATGTGGACAAGTATGCAGTCCCAGTATGAGTTATGGCAGGCTTCTCAA
CAACAACGCCCCGAAATCAGGCCGATATTTGCACATCTATAA

Structures

Toxin

• AlphaFold DB: D3UXL4