Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 31716..32446 Replicon chromosome
Accession NZ_CP097572
Organism Enterobacter kobei strain C210239

Toxin (Protein)


Gene name higB Uniprot ID A0A2W0GI63
Locus tag M8978_RS00150 Protein ID WP_023331686.1
Coordinates 31716..32030 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag M8978_RS00155 Protein ID WP_023331687.1
Coordinates 32027..32446 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M8978_RS00130 (M8978_00130) 27380..28468 + 1089 WP_032635252.1 cellulase family glycosylhydrolase -
M8978_RS00135 (M8978_00135) 28371..29555 - 1185 WP_032637792.1 multidrug efflux MFS transporter EmrD -
M8978_RS00140 (M8978_00140) 29731..30564 - 834 WP_045134488.1 EamA family transporter -
M8978_RS00145 (M8978_00145) 30627..31073 - 447 WP_063614471.1 GNAT family N-acetyltransferase -
M8978_RS00150 (M8978_00150) 31716..32030 + 315 WP_023331686.1 type II toxin-antitoxin system HigB family toxin Toxin
M8978_RS00155 (M8978_00155) 32027..32446 + 420 WP_023331687.1 helix-turn-helix domain-containing protein Antitoxin
M8978_RS00160 (M8978_00160) 32596..32694 + 99 WP_023331688.1 ilvB operon leader peptide IvbL -
M8978_RS00165 (M8978_00165) 32801..34489 + 1689 WP_023331689.1 acetolactate synthase large subunit -
M8978_RS00170 (M8978_00170) 34493..34780 + 288 WP_010426528.1 acetolactate synthase small subunit -
M8978_RS00175 (M8978_00175) 34863..35456 + 594 WP_014881931.1 transcriptional regulator UhpA -
M8978_RS00180 (M8978_00180) 35453..36958 + 1506 WP_045134486.1 signal transduction histidine-protein kinase/phosphatase UhpB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(21-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-136)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12441.59 Da        Isoelectric Point: 10.2825

>T245600 WP_023331686.1 NZ_CP097572:31716-32030 [Enterobacter kobei]
MHLISMKAILDAVIQFPQHREELLFLGRTIEKSHCTTPVALRKIFPTLDNLKYLDKHYVIDIANNNLRVVALIFFESQKF
YVRHVFTHKEYDRFTEKHRTKGKK

Download         Length: 315 bp

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15561.18 Da        Isoelectric Point: 5.6761

>AT245600 WP_023331687.1 NZ_CP097572:32027-32446 [Enterobacter kobei]
MMIVADAMKATHALVAAVPLLGEHPNEKDYQDALELVEYLLMNEPGSPLLDIVCARIRRYEANRPEIVALRQEMESVPVG
IAVLRTLMDQYKLTISDFQDEIGSKSMVSRVLNGQRQLTLNHIKKLAARFGVSPALFIE

Download         Length: 420 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2W0GI63

Antitoxin

Source ID Structure

References