Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 4023573..4024170 Replicon chromosome
Accession NC_013850
Organism Klebsiella variicola At-22

Toxin (Protein)


Gene name higB Uniprot ID -
Locus tag KVAR_RS19020 Protein ID WP_012968756.1
Coordinates 4023853..4024170 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag KVAR_RS19015 Protein ID WP_012542525.1
Coordinates 4023573..4023860 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KVAR_RS18985 4019483..4019731 + 249 WP_008805539.1 DUF1158 domain-containing protein -
KVAR_RS18990 4019748..4020089 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
KVAR_RS18995 4020120..4021235 - 1116 WP_162499993.1 MBL fold metallo-hydrolase -
KVAR_RS19000 4021415..4021996 + 582 WP_012968754.1 TetR/AcrR family transcriptional regulator -
KVAR_RS19005 4021996..4022364 + 369 WP_008805536.1 MmcQ/YjbR family DNA-binding protein -
KVAR_RS19010 4022484..4023137 + 654 WP_012968755.1 oxygen-insensitive NAD(P)H nitroreductase -
KVAR_RS19015 4023573..4023860 - 288 WP_012542525.1 helix-turn-helix transcriptional regulator Antitoxin
KVAR_RS19020 4023853..4024170 - 318 WP_012968756.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
KVAR_RS19025 4024355..4025398 - 1044 WP_012968757.1 DUF2157 domain-containing protein -
KVAR_RS19030 4025867..4026733 - 867 WP_008805530.1 helix-turn-helix transcriptional regulator -
KVAR_RS19035 4026842..4028269 + 1428 WP_012968758.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-89)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12124.41 Da        Isoelectric Point: 11.2767

>T24557 WP_012968756.1 NC_013850:c4024170-4023853 [Klebsiella variicola At-22]
MFRLVVHVDVKKELQALPPIVQAKMIRQIDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
IKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T24557 NC_013850:c4024170-4023853 [Klebsiella variicola At-22]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGCCCATTGTCCAGGCGAAGATGATCCG
GCAAATAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
ATCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10815.51 Da        Isoelectric Point: 10.2212

>AT24557 WP_012542525.1 NC_013850:c4023860-4023573 [Klebsiella variicola At-22]
MSKKIIDWDTLKTELLSQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQILLY

Download         Length: 288 bp

>AT24557 NC_013850:c4023860-4023573 [Klebsiella variicola At-22]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTAGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTGACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCGACGGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGTGGAATAAAGCACCCACAAATTTTACTTTACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References