Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1777412..1777998 Replicon chromosome
Accession NZ_CP097529
Organism Pseudomonas putida strain KT2440 isolate pSEVA631

Toxin (Protein)


Gene name graT Uniprot ID Q88MI5
Locus tag M8Z99_RS08255 Protein ID WP_010952670.1
Coordinates 1777720..1777998 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name graA Uniprot ID Q88MI6
Locus tag M8Z99_RS08250 Protein ID WP_010952669.1
Coordinates 1777412..1777711 (-) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M8Z99_RS08225 (M8Z99_08210) 1772672..1774663 + 1992 WP_010952665.1 sialidase family protein -
M8Z99_RS08230 (M8Z99_08215) 1774675..1775751 + 1077 WP_010952666.1 hypothetical protein -
M8Z99_RS08235 (M8Z99_08220) 1775812..1776255 + 444 WP_049587424.1 structural protein P5 -
M8Z99_RS08240 (M8Z99_08225) 1776252..1776752 + 501 WP_010952668.1 DUF2514 domain-containing protein -
M8Z99_RS08245 (M8Z99_08230) 1776818..1777120 + 303 WP_049587425.1 hypothetical protein -
M8Z99_RS08250 (M8Z99_08235) 1777412..1777711 - 300 WP_010952669.1 type II toxin-antitoxin system antitoxin GraA Antitoxin
M8Z99_RS08255 (M8Z99_08240) 1777720..1777998 - 279 WP_010952670.1 type II toxin-antitoxin system toxin GraT Toxin
M8Z99_RS08260 (M8Z99_08245) 1778249..1779895 + 1647 WP_049587427.1 Na+/H+ antiporter -
M8Z99_RS08265 (M8Z99_08250) 1780001..1781197 - 1197 WP_010952672.1 succinyldiaminopimelate transaminase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(1-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(20-69)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10446.72 Da        Isoelectric Point: 7.2450

>T245542 WP_010952670.1 NZ_CP097529:c1777998-1777720 [Pseudomonas putida]
MIRSFSCADTEALFTTGKTRRGSDIKSVAERKLAMLDAATELRDLRSPPGNRLESLSGNRADQHSIRVNDQWRLCFTWTE
HGPVNVEIVDYH

Download         Length: 279 bp

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10905.55 Da        Isoelectric Point: 5.6793

>AT245542 WP_010952669.1 NZ_CP097529:c1777711-1777412 [Pseudomonas putida]
MLKNGMRPIHPGEILREEFQKEMGFSAAALARALGVATPTVNNILRERGGVSADMALRLSICLDTTPEFWLNLQTAFDLR
TAEQQHGDEIIGSVQRLVA

Download         Length: 300 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6F8S

Antitoxin

Source ID Structure
PDB 6F8S
AlphaFold DB Q88MI6

References