Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HTH(antitoxin)
Location 1373182..1373783 Replicon chromosome
Accession NZ_CP097529
Organism Pseudomonas putida strain KT2440 isolate pSEVA631

Toxin (Protein)


Gene name higB Uniprot ID A0A2L1IAQ2
Locus tag M8Z99_RS06255 Protein ID WP_049587984.1
Coordinates 1373469..1373783 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A2L1IAR7
Locus tag M8Z99_RS06250 Protein ID WP_049587986.1
Coordinates 1373182..1373469 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M8Z99_RS06230 (M8Z99_06215) 1368214..1369059 + 846 WP_003254799.1 DUF6502 family protein -
M8Z99_RS06235 (M8Z99_06220) 1369056..1370774 + 1719 WP_010952348.1 DUF5666 domain-containing protein -
M8Z99_RS06240 (M8Z99_06225) 1370829..1371578 + 750 WP_049587988.1 hypothetical protein -
M8Z99_RS06245 (M8Z99_06230) 1371650..1372981 - 1332 WP_004576169.1 30S ribosomal protein S12 methylthiotransferase RimO -
M8Z99_RS06250 (M8Z99_06235) 1373182..1373469 - 288 WP_049587986.1 helix-turn-helix domain-containing protein Antitoxin
M8Z99_RS06255 (M8Z99_06240) 1373469..1373783 - 315 WP_049587984.1 hypothetical protein Toxin
M8Z99_RS06260 (M8Z99_06245) 1374232..1376142 + 1911 WP_010952353.1 potassium transporter Kup -
M8Z99_RS06265 (M8Z99_06250) 1376191..1377483 - 1293 WP_010952354.1 AcvB/VirJ family lysyl-phosphatidylglycerol hydrolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(36-88)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11784.61 Da        Isoelectric Point: 9.9097

>T245540 WP_049587984.1 NZ_CP097529:c1373783-1373469 [Pseudomonas putida]
MIFIETPVFTSDLKEHLDDEEYRALQAYLAEHPEAGSLLEETGGLRKIRWAAKGKGKSGGVRVIYYHVTAAHQIRMILIY
RKGIVDTLTSSQKAQLRALNKGWK

Download         Length: 315 bp

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10841.31 Da        Isoelectric Point: 7.4063

>AT245540 WP_049587986.1 NZ_CP097529:c1373469-1373182 [Pseudomonas putida]
MEKHLFDRLVESMTQMDEIDRGERQPSREFHVDALQVKKIRQATGLSQAAFAKRIDVAVGTLRNWEQGRREPEGPARALL
RAIHNDPEHVLAALS

Download         Length: 288 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2L1IAQ2

Antitoxin

Source ID Structure
AlphaFold DB A0A2L1IAR7

References