Detailed information of TA system
Bioinformatically predictedOverview
TA module
Type | II | Classification (family/domain) | PP_4151-4152/excise(antitoxin) |
Location | 4690889..4691928 | Replicon | chromosome |
Accession | NZ_CP097528 | ||
Organism | Pseudomonas putida strain KT2440 isolate pMBEC6 |
Toxin (Protein)
Gene name | PP_4152 | Uniprot ID | Q88FE5 |
Locus tag | M8003_RS21750 | Protein ID | WP_010954922.1 |
Coordinates | 4691353..4691928 (+) | Length | 192 a.a. |
Antitoxin (Protein)
Gene name | PP_4151 | Uniprot ID | A0A2V4F8J0 |
Locus tag | M8003_RS21745 | Protein ID | WP_049588231.1 |
Coordinates | 4690889..4691356 (+) | Length | 156 a.a. |
Genomic Context
Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
---|---|---|---|---|---|---|
M8003_RS21730 (M8003_21705) | 4687041..4688114 | + | 1074 | WP_010954918.1 | microcin C ABC transporter permease YejB | - |
M8003_RS21735 (M8003_21710) | 4688116..4689135 | + | 1020 | WP_010954919.1 | ABC transporter permease | - |
M8003_RS21740 (M8003_21715) | 4689137..4690741 | + | 1605 | WP_010954920.1 | ABC transporter ATP-binding protein | - |
M8003_RS21745 (M8003_21720) | 4690889..4691356 | + | 468 | WP_049588231.1 | helix-turn-helix domain-containing protein | Antitoxin |
M8003_RS21750 (M8003_21725) | 4691353..4691928 | + | 576 | WP_010954922.1 | PIN domain-containing protein | Toxin |
M8003_RS21755 (M8003_21730) | 4692142..4695072 | + | 2931 | WP_049588229.1 | aminotransferase | - |
M8003_RS21760 (M8003_21735) | 4695076..4696299 | - | 1224 | WP_010954925.1 | NAD(P)/FAD-dependent oxidoreductase | - |
Associated MGEs
MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
---|---|---|---|---|---|---|---|
inside | Prophage | - | - | 4671245..4695072 | 23827 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 192 a.a. Molecular weight: 21646.86 Da Isoelectric Point: 5.5710
>T245537 WP_010954922.1 NZ_CP097528:4691353-4691928 [Pseudomonas putida]
MKHSPFTAIYDANVLYPAPLRDFLMNLALTGIYRARWSASIHDEWKRNLLLNRPDLTPEHLDRTSSLMDAAVPDALVTDY
DSLVEGLDLPDRDDRHVLAAAIKCNASVIVTFNLKDFSKAVLGAFDIEPLHPDDFIADLWDLDKAAVLEAAQRQRISLKN
PPHSVQQYLDRLLQQKLPETVKLLSAFKFLL
MKHSPFTAIYDANVLYPAPLRDFLMNLALTGIYRARWSASIHDEWKRNLLLNRPDLTPEHLDRTSSLMDAAVPDALVTDY
DSLVEGLDLPDRDDRHVLAAAIKCNASVIVTFNLKDFSKAVLGAFDIEPLHPDDFIADLWDLDKAAVLEAAQRQRISLKN
PPHSVQQYLDRLLQQKLPETVKLLSAFKFLL
Download Length: 576 bp
Antitoxin
Download Length: 156 a.a. Molecular weight: 17112.54 Da Isoelectric Point: 6.3403
>AT245537 WP_049588231.1 NZ_CP097528:4690889-4691356 [Pseudomonas putida]
MTTANLSSHVLPDEKEIAVAVESSRTLAAFLSTKSDTQRIELLNEENQRQVVEVPTFALRLFGEILSELALGNSVKVVPI
HAELTTQEAADLLNVSRPHLVKLLDEAVIPHTKTGRHRRVKFSDLVAYKDKRDGVSRAAIDALAAQAQELKMGYE
MTTANLSSHVLPDEKEIAVAVESSRTLAAFLSTKSDTQRIELLNEENQRQVVEVPTFALRLFGEILSELALGNSVKVVPI
HAELTTQEAADLLNVSRPHLVKLLDEAVIPHTKTGRHRRVKFSDLVAYKDKRDGVSRAAIDALAAQAQELKMGYE
Download Length: 468 bp
Similar Proteins
Only experimentally validated proteins are listed.
Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
---|
Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
---|
Structures
Toxin
Source | ID | Structure |
---|---|---|
AlphaFold DB | Q88FE5 |
Antitoxin
Source | ID | Structure |
---|---|---|
AlphaFold DB | A0A2V4F8J0 |