Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1777567..1778153 Replicon chromosome
Accession NZ_CP097528
Organism Pseudomonas putida strain KT2440 isolate pMBEC6

Toxin (Protein)


Gene name graT Uniprot ID Q88MI5
Locus tag M8003_RS08255 Protein ID WP_010952670.1
Coordinates 1777875..1778153 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name graA Uniprot ID Q88MI6
Locus tag M8003_RS08250 Protein ID WP_010952669.1
Coordinates 1777567..1777866 (-) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M8003_RS08225 (M8003_08210) 1772827..1774818 + 1992 WP_010952665.1 sialidase family protein -
M8003_RS08230 (M8003_08215) 1774830..1775906 + 1077 WP_010952666.1 hypothetical protein -
M8003_RS08235 (M8003_08220) 1775967..1776410 + 444 WP_049587424.1 structural protein P5 -
M8003_RS08240 (M8003_08225) 1776407..1776907 + 501 WP_010952668.1 DUF2514 domain-containing protein -
M8003_RS08245 (M8003_08230) 1776973..1777275 + 303 WP_049587425.1 hypothetical protein -
M8003_RS08250 (M8003_08235) 1777567..1777866 - 300 WP_010952669.1 type II toxin-antitoxin system antitoxin GraA Antitoxin
M8003_RS08255 (M8003_08240) 1777875..1778153 - 279 WP_010952670.1 type II toxin-antitoxin system toxin GraT Toxin
M8003_RS08260 (M8003_08245) 1778404..1780050 + 1647 WP_049587427.1 Na+/H+ antiporter -
M8003_RS08265 (M8003_08250) 1780156..1781352 - 1197 WP_010952672.1 succinyldiaminopimelate transaminase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(1-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(20-69)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10446.72 Da        Isoelectric Point: 7.2450

>T245533 WP_010952670.1 NZ_CP097528:c1778153-1777875 [Pseudomonas putida]
MIRSFSCADTEALFTTGKTRRGSDIKSVAERKLAMLDAATELRDLRSPPGNRLESLSGNRADQHSIRVNDQWRLCFTWTE
HGPVNVEIVDYH

Download         Length: 279 bp

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10905.55 Da        Isoelectric Point: 5.6793

>AT245533 WP_010952669.1 NZ_CP097528:c1777866-1777567 [Pseudomonas putida]
MLKNGMRPIHPGEILREEFQKEMGFSAAALARALGVATPTVNNILRERGGVSADMALRLSICLDTTPEFWLNLQTAFDLR
TAEQQHGDEIIGSVQRLVA

Download         Length: 300 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6F8S

Antitoxin

Source ID Structure
PDB 6F8S
AlphaFold DB Q88MI6

References