Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 1449221..1450011 Replicon chromosome
Accession NZ_CP097528
Organism Pseudomonas putida strain KT2440 isolate pMBEC6

Toxin (Protein)


Gene name yhaV Uniprot ID Q88NE5
Locus tag M8003_RS06615 Protein ID WP_010952397.1
Coordinates 1449221..1449688 (-) Length 156 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID I7C311
Locus tag M8003_RS06620 Protein ID WP_014859917.1
Coordinates 1449685..1450011 (-) Length 109 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M8003_RS06595 (M8003_06580) 1444384..1445913 + 1530 WP_010952395.1 efflux transporter outer membrane subunit -
M8003_RS06600 (M8003_06585) 1445910..1448087 + 2178 WP_003251716.1 FUSC family protein -
M8003_RS06605 (M8003_06590) 1448077..1448277 + 201 WP_003251718.1 DUF1656 domain-containing protein -
M8003_RS06610 (M8003_06595) 1448288..1449148 + 861 WP_010952396.1 HlyD family secretion protein -
M8003_RS06615 (M8003_06600) 1449221..1449688 - 468 WP_010952397.1 type II toxin-antitoxin system YhaV family toxin Toxin
M8003_RS06620 (M8003_06605) 1449685..1450011 - 327 WP_014859917.1 type II toxin-antitoxin system PrlF family antitoxin Antitoxin
M8003_RS06625 (M8003_06610) 1450104..1450550 - 447 WP_010952399.1 universal stress protein -
M8003_RS06630 (M8003_06615) 1450824..1451729 + 906 WP_010952400.1 LysR family transcriptional regulator -
M8003_RS06635 (M8003_06620) 1451846..1453387 + 1542 WP_010952401.1 MFS transporter -
M8003_RS06640 (M8003_06625) 1453416..1454477 + 1062 WP_049587435.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(17-152)

Antitoxin

PrlF antitoxin

(8-106)


Sequences

Toxin        


Download         Length: 156 a.a.        Molecular weight: 17962.34 Da        Isoelectric Point: 9.7963

>T245532 WP_010952397.1 NZ_CP097528:c1449688-1449221 [Pseudomonas putida]
MSDASRKPLVIHGWTVIAHPLFLAKLDALSAQVEAQRDKDPTGYTKRNTFKRLAAIRRLAFDVIPQDPTKPEYRQGATLG
GDHKHWFRAKFFQQYRLFFRYHTPSRIIVLAWINDESSKRAYESSDDAYKVFQKMLHSGNPPDDWDQLLQEAAAD

Download         Length: 468 bp

Antitoxin


Download         Length: 109 a.a.        Molecular weight: 11919.40 Da        Isoelectric Point: 4.3722

>AT245532 WP_014859917.1 NZ_CP097528:c1450011-1449685 [Pseudomonas putida]
MAATFDVESTLTDRYQTTVPETVRRALGLKKRDKIHYSIRPDGGVILTRAEATEDDPVLGKFLDFLAHDIATNPQRLQVV
DAGLIARLDKLVGDAEVDLDQPLSADDE

Download         Length: 327 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q88NE5

Antitoxin

Source ID Structure
AlphaFold DB I7C311

References