Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mqsRA (relBE)/MqsR-MqsA
Location 4750064..4750764 Replicon chromosome
Accession NZ_CP097527
Organism Pseudomonas putida strain KT2440 isolate pnCas9-BEC6-C

Toxin (Protein)


Gene name mqsR Uniprot ID Q88F93
Locus tag M8001_RS22015 Protein ID WP_010954960.1
Coordinates 4750468..4750764 (-) Length 99 a.a.

Antitoxin (Protein)


Gene name mqsA Uniprot ID I7AUJ7
Locus tag M8001_RS22010 Protein ID WP_003254235.1
Coordinates 4750064..4750465 (-) Length 134 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M8001_RS21990 (M8001_21965) 4745198..4746019 - 822 WP_010954957.1 transporter substrate-binding domain-containing protein -
M8001_RS21995 (M8001_21970) 4746095..4747024 - 930 WP_010954958.1 FAD-binding protein -
M8001_RS22000 (M8001_21975) 4747025..4747774 - 750 WP_003254232.1 electron transfer flavoprotein subunit beta/FixA family protein -
M8001_RS22005 (M8001_21980) 4748310..4749992 + 1683 WP_010954959.1 electron transfer flavoprotein-ubiquinone oxidoreductase -
M8001_RS22010 (M8001_21985) 4750064..4750465 - 402 WP_003254235.1 type II toxin-antitoxin system MqsA family antitoxin Antitoxin
M8001_RS22015 (M8001_21990) 4750468..4750764 - 297 WP_010954960.1 type II toxin-antitoxin system MqsR family toxin Toxin
M8001_RS22020 (M8001_21995) 4750859..4752184 - 1326 WP_010954961.1 MFS transporter -
M8001_RS22025 (M8001_22000) 4752257..4752736 - 480 WP_010954962.1 hypothetical protein -
M8001_RS22030 (M8001_22005) 4752905..4753381 - 477 WP_010954963.1 sigma-70 family RNA polymerase sigma factor -
M8001_RS22035 (M8001_22010) 4753594..4754772 + 1179 WP_010954964.1 efflux RND transporter periplasmic adaptor subunit -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin MqsR

(6-97)

Antitoxin

Cro/C1-type helix-turn-helix domain

(74-109)


Sequences

Toxin        


Download         Length: 99 a.a.        Molecular weight: 11133.22 Da        Isoelectric Point: 10.2271

>T245529 WP_010954960.1 NZ_CP097527:c4750764-4750468 [Pseudomonas putida]
MEKRMPHCPLERVKALAAARRIRPTGAALKGAKALGMDFPGMLEVITSLKRTDFYKSMTSHIDHRVWQDVYRPLTAIGYV
YLKLSVVDDVLIVSFKEL

Download         Length: 297 bp

Antitoxin


Download         Length: 134 a.a.        Molecular weight: 14684.76 Da        Isoelectric Point: 4.9531

>AT245529 WP_003254235.1 NZ_CP097527:c4750465-4750064 [Pseudomonas putida]
MRCPICGGSELAPDIQGMPYSYKGEMTVIPEVSGDYCSACGECVLSHDEAMRVSHLMTAFERQVNANVVDPSFIASIRRK
FDLDQREAGEIFGGGVNAFSRYENGKTTPPVALVKLLKLLDRHPELFEEVRTA

Download         Length: 402 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q88F93

Antitoxin

Source ID Structure
AlphaFold DB I7AUJ7

References