Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PP_4151-4152/excise(antitoxin)
Location 4690886..4691925 Replicon chromosome
Accession NZ_CP097527
Organism Pseudomonas putida strain KT2440 isolate pnCas9-BEC6-C

Toxin (Protein)


Gene name PP_4152 Uniprot ID Q88FE5
Locus tag M8001_RS21750 Protein ID WP_010954922.1
Coordinates 4691350..4691925 (+) Length 192 a.a.

Antitoxin (Protein)


Gene name PP_4151 Uniprot ID A0A2V4F8J0
Locus tag M8001_RS21745 Protein ID WP_049588231.1
Coordinates 4690886..4691353 (+) Length 156 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M8001_RS21730 (M8001_21705) 4687038..4688111 + 1074 WP_010954918.1 microcin C ABC transporter permease YejB -
M8001_RS21735 (M8001_21710) 4688113..4689132 + 1020 WP_010954919.1 ABC transporter permease -
M8001_RS21740 (M8001_21715) 4689134..4690738 + 1605 WP_010954920.1 ABC transporter ATP-binding protein -
M8001_RS21745 (M8001_21720) 4690886..4691353 + 468 WP_049588231.1 helix-turn-helix domain-containing protein Antitoxin
M8001_RS21750 (M8001_21725) 4691350..4691925 + 576 WP_010954922.1 PIN domain-containing protein Toxin
M8001_RS21755 (M8001_21730) 4692139..4695069 + 2931 WP_049588229.1 aminotransferase -
M8001_RS21760 (M8001_21735) 4695073..4696296 - 1224 WP_010954925.1 NAD(P)/FAD-dependent oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 4671242..4695069 23827


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(9-115)

Antitoxin

Helix-turn-helix domain, group 17

(84-132)


Sequences

Toxin        


Download         Length: 192 a.a.        Molecular weight: 21646.86 Da        Isoelectric Point: 5.5710

>T245528 WP_010954922.1 NZ_CP097527:4691350-4691925 [Pseudomonas putida]
MKHSPFTAIYDANVLYPAPLRDFLMNLALTGIYRARWSASIHDEWKRNLLLNRPDLTPEHLDRTSSLMDAAVPDALVTDY
DSLVEGLDLPDRDDRHVLAAAIKCNASVIVTFNLKDFSKAVLGAFDIEPLHPDDFIADLWDLDKAAVLEAAQRQRISLKN
PPHSVQQYLDRLLQQKLPETVKLLSAFKFLL

Download         Length: 576 bp

Antitoxin


Download         Length: 156 a.a.        Molecular weight: 17112.54 Da        Isoelectric Point: 6.3403

>AT245528 WP_049588231.1 NZ_CP097527:4690886-4691353 [Pseudomonas putida]
MTTANLSSHVLPDEKEIAVAVESSRTLAAFLSTKSDTQRIELLNEENQRQVVEVPTFALRLFGEILSELALGNSVKVVPI
HAELTTQEAADLLNVSRPHLVKLLDEAVIPHTKTGRHRRVKFSDLVAYKDKRDGVSRAAIDALAAQAQELKMGYE

Download         Length: 468 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q88FE5

Antitoxin

Source ID Structure
AlphaFold DB A0A2V4F8J0

References