Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3707744..3708285 Replicon chromosome
Accession NZ_CP097430
Organism Escherichia coli strain C288

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag M8Y68_RS18180 Protein ID WP_000615983.1
Coordinates 3708007..3708285 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag M8Y68_RS18175 Protein ID WP_000729703.1
Coordinates 3707744..3708004 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M8Y68_RS18155 (3703419) 3703419..3704204 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
M8Y68_RS18160 (3704176) 3704176..3705888 + 1713 Protein_3570 flagellar biosynthesis protein FlhA -
M8Y68_RS18165 (3706112) 3706112..3706609 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
M8Y68_RS18170 (3706785) 3706785..3707534 - 750 WP_000056849.1 C40 family peptidase -
M8Y68_RS18175 (3707744) 3707744..3708004 + 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
M8Y68_RS18180 (3708007) 3708007..3708285 + 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
M8Y68_RS18185 (3708441) 3708441..3709181 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
M8Y68_RS18190 (3709152) 3709152..3709919 - 768 WP_000333380.1 class II glutamine amidotransferase -
M8Y68_RS18195 (3710125) 3710125..3710703 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T245482 WP_000615983.1 NZ_CP097430:3708007-3708285 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT245482 WP_000729703.1 NZ_CP097430:3707744-3708004 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References