Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3701547..3702241 Replicon chromosome
Accession NZ_CP097430
Organism Escherichia coli strain C288

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag M8Y68_RS18140 Protein ID WP_001263489.1
Coordinates 3701547..3701945 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag M8Y68_RS18145 Protein ID WP_000554758.1
Coordinates 3701948..3702241 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- (3697135) 3697135..3697215 - 81 NuclAT_11 - -
- (3697135) 3697135..3697215 - 81 NuclAT_11 - -
- (3697135) 3697135..3697215 - 81 NuclAT_11 - -
- (3697135) 3697135..3697215 - 81 NuclAT_11 - -
M8Y68_RS18115 (3697811) 3697811..3698269 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
M8Y68_RS18120 (3698530) 3698530..3699987 + 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
M8Y68_RS18125 (3700044) 3700044..3700565 - 522 Protein_3563 peptide chain release factor H -
M8Y68_RS18130 (3700561) 3700561..3700767 - 207 Protein_3564 RtcB family protein -
M8Y68_RS18135 (3701085) 3701085..3701537 - 453 WP_001059892.1 GNAT family N-acetyltransferase -
M8Y68_RS18140 (3701547) 3701547..3701945 - 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
M8Y68_RS18145 (3701948) 3701948..3702241 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
M8Y68_RS18150 (3702293) 3702293..3703348 - 1056 WP_001226164.1 DNA polymerase IV -
M8Y68_RS18155 (3703419) 3703419..3704204 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
M8Y68_RS18160 (3704176) 3704176..3705888 + 1713 Protein_3570 flagellar biosynthesis protein FlhA -
M8Y68_RS18165 (3706112) 3706112..3706609 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T245481 WP_001263489.1 NZ_CP097430:c3701945-3701547 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT245481 WP_000554758.1 NZ_CP097430:c3702241-3701948 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References