Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3693850..3694544 Replicon chromosome
Accession NZ_CP097426
Organism Escherichia coli strain C289

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag M8Y67_RS18090 Protein ID WP_001263489.1
Coordinates 3693850..3694248 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag M8Y67_RS18095 Protein ID WP_000554758.1
Coordinates 3694251..3694544 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- (3689438) 3689438..3689518 - 81 NuclAT_11 - -
- (3689438) 3689438..3689518 - 81 NuclAT_11 - -
- (3689438) 3689438..3689518 - 81 NuclAT_11 - -
- (3689438) 3689438..3689518 - 81 NuclAT_11 - -
M8Y67_RS18065 (3690114) 3690114..3690572 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
M8Y67_RS18070 (3690833) 3690833..3692290 + 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
M8Y67_RS18075 (3692347) 3692347..3692868 - 522 Protein_3554 peptide chain release factor H -
M8Y67_RS18080 (3692864) 3692864..3693070 - 207 Protein_3555 RtcB family protein -
M8Y67_RS18085 (3693388) 3693388..3693840 - 453 WP_001059892.1 GNAT family N-acetyltransferase -
M8Y67_RS18090 (3693850) 3693850..3694248 - 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
M8Y67_RS18095 (3694251) 3694251..3694544 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
M8Y67_RS18100 (3694596) 3694596..3695651 - 1056 WP_001226164.1 DNA polymerase IV -
M8Y67_RS18105 (3695722) 3695722..3696507 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
M8Y67_RS18110 (3696479) 3696479..3698191 + 1713 Protein_3561 flagellar biosynthesis protein FlhA -
M8Y67_RS18115 (3698415) 3698415..3698912 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T245455 WP_001263489.1 NZ_CP097426:c3694248-3693850 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT245455 WP_000554758.1 NZ_CP097426:c3694544-3694251 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References