Detailed information of TA system TA024545

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 341642..342288
• Replicon: chromosome
• Accession: NC_013850
• Organism: Klebsiella variicola At-22

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: KVAR_RS01525
• Protein ID: WP_012967128.1
• Coordinates: 341642..341989 (+)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A1F2LZT5
• Locus tag: KVAR_RS01530
• Protein ID: WP_008806992.1
• Coordinates: 341989..342288 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KVAR_RS01515	337529..338962	+	1434	WP_004202133.1	glycogen synthase GlgA	-
KVAR_RS01520	338980..341427	+	2448	WP_008806990.1	glycogen phosphorylase	-
KVAR_RS01525	341642..341989	+	348	WP_012967128.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KVAR_RS01530	341989..342288	+	300	WP_008806992.1	helix-turn-helix transcriptional regulator	Antitoxin
KVAR_RS01535	342351..343859	-	1509	WP_008806993.1	glycerol-3-phosphate dehydrogenase	-
KVAR_RS01540	344064..344393	+	330	WP_004202138.1	thiosulfate sulfurtransferase GlpE	-
KVAR_RS01545	344444..345274	+	831	WP_008806994.1	rhomboid family intramembrane serine protease GlpG	-
KVAR_RS01550	345324..346082	+	759	WP_008806995.1	DeoR/GlpR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-107)

Antitoxin

• Cro/C1-type helix-turn-helix domain (31-84)

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 13506.49 Da
• Isoelectric Point: 5.6651

>T24545 WP_012967128.1 NC_013850:341642-341989 [Klebsiella variicola At-22]
MWDVETTDTFDAWFELQSRALKEDMLATMLILSEFGPQLGRPYVDTVKDSTFQNMKELWAQHHGLPIRAFFAFDPLRKAI
VLCAGNKDGMNEKRFYKEMITLADREFSQHLTKER

Nucleotide

• Length: 348 bp

>T24545 NC_013850:341642-341989 [Klebsiella variicola At-22]
ATGTGGGATGTAGAAACGACGGATACGTTTGATGCCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCTTGTCTGAGTTTGGTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTCTGGGCTCAGCATCATGGGCTCCCCATCCGCGCCTTTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGCGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10814.61 Da
• Isoelectric Point: 10.1464

>AT24545 WP_008806992.1 NC_013850:341989-342288 [Klebsiella variicola At-22]
MATLKELMAKQSPESQQRIAAKAAEIRQSVALNLLREELQMSQTEMAAAMGVKQPTIAKMEQADNDPRLSTLKRYIAALG
GELSINVTLPTGKKVSFNL

Nucleotide

• Length: 300 bp

>AT24545 NC_013850:341989-342288 [Klebsiella variicola At-22]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGAAAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCTGATAACGATCCGCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACGTTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A1F2LZT5